Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening
Identification of proteins interacting with Toxoplasma SRCAP by yeast two-hybrid screening
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DOI：
10.1007/s00436-004-1291-5
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发表时间：
2005-03-01
影响因子：
2
通讯作者：
Sullivan, WJ
中科院分区：
文献类型：
--
作者：
Nallani, KC;Sullivan, WJ
Toxoplasma gondii is an opportunistic protozoan parasite that differentiates into latent cysts (bradyzoite) that can be reactivated during immunosuppression. TgSRCAP (Toxoplasma gondii Snf2-related CBP activator protein) is a SWI2/SNF2 family chromatin remodeler whose expression increases during cyst development. Identifying the proteins associating with TgSRCAP during the pre-cyst stage (tachyzoite) will increase our understanding of how parasite differentiation is initiated. We employed the yeast two-hybrid system to identify proteins that may interact directly with TgSRCAP. A stretch of 1,060 amino acids between ATPase subdomains IV and V of TgSRCAP was chosen as "bait" since the corresponding region in human SRCAP interacts with other proteins, including CREB binding protein. We have identified several novel parasite-specific transcription factors predicted to be in the T. gondii genome. Metabolic enzymes that may participate in cyst development were also identified as interacting with TgSRCAP.
