Sonic Hedgehog Regulates Bone Fracture Healing
Sonic Hedgehog Regulates Bone Fracture Healing
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DOI：
10.3390/ijms21020677
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发表时间：
2020-01-02
影响因子：
5.6
通讯作者：
Irie, Kazuharu
中科院分区：
文献类型：
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作者：
Takebe, Hiroaki;Shalehin, Nazmus;Irie, Kazuharu
骨折愈合涉及膜内和软骨内骨化的结合。众所周知，印度刺猬(IHH)在骨折愈合过程中促进软骨生成。同时，参与个体发育的Sonic Hedgehog(Shh)被报道参与骨折愈合，但细节尚未澄清。在这项研究中，我们证明Shh参与了骨折的愈合。本研究选用6周龄SD大鼠和Gli-Creer(T2)；td番茄小鼠。实验动物右侧肋骨骨折。检测Shh和Gli1在骨折愈合过程中的定位。Gli1子代细胞和OSX阳性细胞在骨折愈合过程中的定位相似。骨折后第7天，新生骨基质和软骨细胞表面均可见成骨细胞标志物矮小相关转录因子2(Runx2)和OSX。Shh和Gli1与Runx2和OSx共定位。这些发现提示Shh参与了骨折愈合过程中的膜内和软骨内骨化。
Bone fracture healing involves the combination of intramembranous and endochondral ossification. It is known that Indian hedgehog (Ihh) promotes chondrogenesis during fracture healing. Meanwhile, Sonic hedgehog (Shh), which is involved in ontogeny, has been reported to be involved in fracture healing, but the details had not been clarified. In this study, we demonstrated that Shh participated in fracture healing. Six-week-old Sprague-Dawley rats and Gli-CreER(T2); tdTomato mice were used in this study. The right rib bones of experimental animals were fractured. The localization of Shh and Gli1 during fracture healing was examined. The localization of Gli1 progeny cells and osterix (Osx)-positive cells was similar during fracture healing. Runt-related transcription factor 2 (Runx2) and Osx, both of which are osteoblast markers, were observed on the surface of the new bone matrix and chondrocytes on day seven after fracture. Shh and Gli1 were co-localized with Runx2 and Osx. These findings suggest that Shh is involved in intramembranous and endochondral ossification during fracture healing.