Trypanosoma cruzi: in vitro induction of macrophage microbicidal activity
Trypanosoma cruzi: in vitro induction of macrophage microbicidal activity
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克氏锥虫：体外诱导巨噬细胞杀菌活性
DOI：
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发表时间：
1978
影响因子：
15.3
通讯作者：
Z. Cohn
中科院分区：
文献类型：
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作者：
N. Nogueira;Z. Cohn
通过暴露于抗原脉冲、致敏的脾细胞群的产物，可以诱导正常、常驻和炎症小鼠腹腔巨噬细胞对克氏锥虫的锥乳鞭毛虫显示杀微生物活性。通过持续暴露和每日更新脾脏细胞因子，可以获得最佳的巨噬细胞杀微生物活性。腹腔注射轻度炎症剂后获得的巨噬细胞被迅速诱导，暴露于活性脾细胞因子24小时后显示出锥虫活性，48小时后不再观察到寄生虫。常驻腹膜巨噬细胞需要24小时以上的活化时间。在实现细胞内寄生虫完全消失之前去除该因子导致存活生物体恢复生长。脾细胞因子在巨噬细胞暴露于锥乳线虫之前或之后添加都是有效的，并且本身不会改变寄生虫的生存能力。将该因子稀释至1:16仍可产生显著的锥虫活性。体内激活的细胞，在感染克氏锥虫或卡尔梅特- guerin杆菌的动物经过特定的二次攻击后获得，在体外条件下失去了它们的锥虫杀灭活性。这种活性的丧失可以通过添加活性脾细胞因子来预防或恢复。用刀豆蛋白A或脂多糖刺激的正常脾细胞的产物也可诱导锥虫活性。
Normal, resident and inflammatory mouse peritoneal macrophages can be induced to display microbicidal activity against trypomastigotes of Trypanosoma cruzi by exposure to products from antigen-pulsed, sensitized spleen cell populations. Optimal macrophage microbicidal activity was achieved by constant exposure and daily renewal of the spleen cell factors. Macrophages obtained after an intraperitoneal injection of mild inflammatory agents were rapidly induced, displaying trypanocidal activity 24 h after exposure to the active spleen cell factor(s), and by 48 h, parasites were no longer observed. Resident peritoneal macrophages required 24 h longer for activation. Removal of the factor(s) before achieving complete disappearance of intracellular parasites led to resumed growth of the surviving organisms. The spleen cell factor(s) is effective when added either before or after exposure of the macrophages to trypomastigotes, and does not itself alter parasite viability. Dilution of the factor(s) up to 1:16 still results in significant trypanocidal activity. In vivo activated cells, obtained after a specific secondary challenge of animals infected with T. cruzi or Bacille Calmette-Guerin, lose their trypanocidal activity under in vitro conditions. This loss of activity can be prevented or restored by the addition of the active spleen cell factor(s). Induction of trypanocidal activity is also obtained with products from Concanavalin A- or lipopolysaccharide-stimulated normal spleen cells.