Increased expression of calcineurin in human colorectal adenocarcinomas
Increased expression of calcineurin in human colorectal adenocarcinomas
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DOI：
10.1002/jcb.20437
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发表时间：
2005-07-01
影响因子：
4
通讯作者：
Sharma, RK
中科院分区：
文献类型：
--
作者：
Lakshmikuttyamma, A;Selvakumar, P;Sharma, RK
结直肠癌（CRC）是西方世界癌症死亡的第三大常见原因。钙调神经磷酸酶（CaN）是一种依赖于钙/钙调素（CaM）的蛋白磷酸酶，在钙离子介导的信号转导中起重要作用。本研究的主要目的是研究钙/钙调素依赖性蛋白磷酸酶在正常和侵袭性肿瘤成分的人类样本中的潜在作用。在这项研究中，我们进行了45例CaN活性，13例CaN蛋白表达的Western印迹分析，6例免疫组化分析在正常和侵袭性肿瘤成分的人类样本。免疫组化分析显示，不同强度的强细胞质染色观察到所有患者的结肠肿瘤相比，正常粘膜。此外，蛋白质印迹分析揭示了一个突出的过表达的免疫反应性条带，其表观分子量为60 kDa催化α亚基（CaN A）以及结肠肿瘤样品中的CaN A α和β。CaN蛋白表达升高似乎是Ca 2+信号传导和致癌过程之间的可能联系。(c)2005年Wiley-Liss，Inc.
Colorectal cancer (CRC) is the third most common cause of cancer death in the Western world. Calcineurin (CaN), a Ca2+/Calimodulin (CaM)-dependent protein phosphatase, is important for Ca2+-mediated signal transduction. The main objective of this study is to examine the potential role of Ca2+/CaM-dependent protein phosphatase in both normal and in invasive tumor components of human samples. In this study, we carried out 45 cases of CaN activity, 13 cases of CaN protein expression by Western blot analysis, and 6 cases for immunohistochemical analysis in both normal and invasive tumor components of human samples. Immunohistochemical analysis revealed that strong cytoplasmic staining of varying intensity was observed in colon tumors of all patients compared to normal mucosa. In addition, Western blot analysis revealed a prominent overexpressed immunoreactive band with an apparent molecular mass of 60 kDa catalytic alpha subunit (CaN A) as well as CaN A alpha and beta in colon tumor samples. Elevated CaN protein expression appears to be a possible link between Ca2+ signaling and oncogenic processes. (c) 2005 Wiley-Liss, Inc.