Comparison of dynamic FDG-microPET study in a rabbit turpentine-induced inflammatory model and in a rabbit VX2 tumor model
Comparison of dynamic FDG-microPET study in a rabbit turpentine-induced inflammatory model and in a rabbit VX2 tumor model
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DOI：
10.1007/bf03033999
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Inoue, Yuichi
中科院分区：
文献类型：
--
作者：
Hamazawa, Yoshimasa;Koyama, Koichi;Inoue, Yuichi
目的：我们研究了在动物模型中使用MicroPET P4扫描仪获得的动态FDG-microPET扫描区分肿瘤和炎症的最佳时间。材料与方法：采用家兔46只，共92个炎性病灶，分别于注射松节油0.2ml（组1）或1.0ml（组2）后2、5、7、14、30和60 d建立炎症模型。采用5只兔共10个VX 2肿瘤作为肿瘤模型。在PET研究之前进行螺旋CT扫描。在PET研究中，禁食4小时后，进行透射扫描和动态发射数据采集，直至静脉注射FDG后2小时。使用滤波反投影法每10分钟重建一次图像。参照CT图像对PET图像进行视觉分析。对于定量分析，在参考CT图像在肿瘤和肌肉中设定感兴趣区域之后计算炎症-肌肉（I/M）比率和肿瘤-肌肉（T/M）比率，并且制备时间-I/M比率和时间-T/M比率曲线（TRC）以显示这些比率随时间的变化。检查炎性病变和肿瘤病变的组织学表现，并与CT和FDG-microPET图像进行比较。结果如下：在目视和定量分析中，除第1组第60天显示几乎平坦的曲线外，所有I/M比和T/M比均随时间增加。T/M比值的TRC随时间呈线性增加，而I/M比值的TRC随时间最多呈抛物线增加。FDG在炎性病变中的摄取反映了组织学结果。双时相显像早期显像40 min时，延迟显像必须在早期显像后30 min进行，而单时相显像需在静脉注射FDG后90 min或更晚进行。结论：我们的研究结果表明，在临床实践中，采用双时间点成像，而不是单时间点成像，缩短整体测试时间的可能性。
Purpose: We investigated the optimum time for the differentiation tumor from inflammation using dynamic FDG-microPET scans obtained by a MicroPET P4 scanner in animal models. Materials and Methods: Forty-six rabbits with 92 inflammatory lesions that were induced 2, 5, 7, 14, 30 and 60 days after 0.2 ml (Group 1) or 1.0 ml (Group 2) of turpentine oil injection were used as inflammatory models. Five rabbits with 10 VX2 tumors were used as the tumor model. Helical CT scans were performed before the PET studies. In the PET study, after 4 hours fasting, and following transmission scans and dynamic emission data acquisitions were performed until 2 hours after intravenous FDG injection. Images were reconstructed every 10 minutes using a filtered-back projection method. PET images were analyzed visually referring to CT images. For quantitative analysis, the inflammation-to-muscle (I/M) ratio and tumor-to-muscle (T/M) ratio were calculated after regions of interest were set in tumors and muscles referring to CT images and the time-I/M ratio and time-T/M ratio curves (TRCs) were prepared to show the change over time in these ratios. The histological appearance of both inflammatory lesions and tumor lesions were examined and compared with the CT and FDG-microPET images. Results: In visual and quantitative analysis, All the I/M ratios and the T/M ratios increased over time except that Day 60 of Group 1 showed an almost flat curve. The TRC of the T/M ratio showed a linear increasing curve over time, while that of the I/M ratios showed a parabolic increasing over time at the most. FDG uptake in the inflammatory lesions reflected the histological findings. For differentiating tumors from inflammatory lesions with the early image acquired at 40 min for dual-time imaging, the delayed image must be acquired 30 min after the early image, while imaging at 90 min or later after intravenous FDG injection was necessary in single-time-point imaging. Conclusion: Our results suggest the possibility of shortening the overall testing time in clinical practice by adopting dual-time-point imaging rather than single-time-point imaging.