Highly-porous diatom biosilica stationary phase for thin-layer chromatography
Highly-porous diatom biosilica stationary phase for thin-layer chromatography
复制标题
DOI：
10.1016/j.chroma.2019.01.037
复制
发表时间：
2019-04-26
影响因子：
4.1
通讯作者：
Wang, Alan X.
中科院分区：
文献类型：
--
作者：
Kraai, Joseph A.;Rorrer, Gregory L.;Wang, Alan X.
这项研究表明，纳米结构，高度多孔的固定相组成的随机沉积的生物硅硅藻壳分离硅藻羽纹藻的活细胞。显着提高了传统的薄层色谱法（TLC）的基础上分离的三苯甲烷染料孔雀石绿色和快速绿色相对于硅胶的两个移动的相（9：1：1 v/v 1-丁醇：乙醇：水，5：1：2 v/v 1-丁醇：乙酸：水）。虽然这两种固定相组成的无定形二氧化硅富含硅烷醇基团的粒径为10-12 μ m的硅藻生物硅硅藻壳是高度多孔的，中空的壳与表面结构为主的200 nm的孔阵列。硅藻生物硅显着提高了孔雀石绿色和快速绿色的流动性，使这些分析物的分辨率。硅藻生物二氧化硅层具有96%的高空隙率，但相对于硅胶，流速和渗透常数降低了两倍。TLC性能得到增强，如使用1-丁醇：乙酸：水移动的相时两种分析物的理论塔板高度降低10倍，以及使用1-丁醇：乙醇：水移动的相时孔雀石绿色和固绿色之间保留时间差异增加（Δ R-f = 0.26）所证明。通过修改的货车Deemter方程分析板高度与溶剂前沿位置表明，分散传质减少，导致分析物分辨率提高，并且硅藻壳表面降低了边界层阻力，导致分析物通量增加。总的来说，改进色谱性能的基础被认为是硅藻生物二氧化硅硅藻壳独特的纳米和微观结构。(C)2019爱思唯尔B. V.保留所有权利。
This study showed that a nanostructured, highly-porous stationary phase composed of randomly deposited biosilica frustules isolated from living cells of diatom Pinnularia sp. significantly improved the conventional thin-layer chromatography (TLC) based separation of the triphenylmethane dyes malachite green and fast green relative to silica gel by two mobile phases (9:1:1 v/v 1-butanol:ethanol:water, 5:1:2 v/v 1-butanol:acetic acid:water). Although both stationary phases were composed of amorphous silica rich in silanol groups with particle size of 10-12 mu m diatom biosilica frustules were highly porous, hollow shells with surface structure dominated by 200 nm pore arrays. Diatom biosilica significantly improved the mobility of both malachite green and fast green, enabling the resolution of these analytes. The diatom biosilica layer had a high void fraction of 96% but reduced the flow velocity and permeability constant by a factor of two relative to silica gel. TLC performance was enhanced, as evidenced by tenfold reduction in theoretical plate height for both analytes using the 1-butanol:acetic acid:water mobile phase, and an increased difference in retention time between malachite green and fast green (Delta R-f = 0.26) using the 1-butanol:ethanol:water mobile phase. Analysis of plate height vs. solvent front position by the modified van Deemter equation suggested that dispersive mass transfer was reduced, leading to improved analyte resolution, and that surface of the frustule decreased boundary layer resistance, leading to increased analyte flux. Overall, the basis for improved chromatographic performance is believed to be the unique nano- and microstructure of the diatom biosilica frustule. (C) 2019 Elsevier B.V. All rights reserved.