High-resolution view of bacteriophage lambda gene expression by ribosome profiling
High-resolution view of bacteriophage lambda gene expression by ribosome profiling
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DOI：
10.1073/pnas.1309739110
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发表时间：
2013-07-16
影响因子：
11.1
通讯作者：
Roberts, Jeffrey W.
中科院分区：
文献类型：
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作者：
Liu, Xiaoqiu;Jiang, Huifeng;Roberts, Jeffrey W.
λ噬菌体是研究最广泛的生物体之一，并且一直是理解遗传调控基本模式的主要模型。在这里，我们研究的进展，λ基因表达噬菌体发育过程中的核糖体分析，从而提供了一个非常高分辨率的λ基因表达。已知基因以可预测的方式表达，验证了分析。然而，许多以前未被认识到的潜在开放阅读框架在表达分析中变得明显，揭示了lambda基因功能模式的意外复杂性。
Bacteriophage lambda is one of the most extensively studied organisms and has been a primary model for understanding basic modes of genetic regulation. Here, we examine the progress of lambda gene expression during phage development by ribosome profiling and, thereby, provide a very-high-resolution view of lambda gene expression. The known genes are expressed in a predictable fashion, authenticating the analysis. However, many previously unappreciated potential open reading frames become apparent in the expression analysis, revealing an unexpected complexity in the pattern of lambda gene function.