Single-hair analysis of zolpidem on the supposition of its single administration in drug-facilitated crimes
Single-hair analysis of zolpidem on the supposition of its single administration in drug-facilitated crimes
复制标题
DOI：
10.1007/s11419-014-0260-7
复制
发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Suzuki, Koichi
中科院分区：
文献类型：
--
作者：
Shima, Noriaki;Sasaki, Keiko;Suzuki, Koichi
关于单次给药后掺入头发中的安眠药的量以及记录药物性侵犯等案件的有效分析程序的信息很少。为了获得基本信息，使用一锅粉碎提取方法，通过新建立的液相色谱-串联质谱程序对服用单剂量 10 mg 唑吡坦 (ZP) 的志愿者的单根头发样本进行了分析。 ZP 的检测限为 50 fg/2-cm 单根头发，并且针对单根黑发样本（n = 15）确定每个片段的 ZP。在 14 根毛发中可检测到 ZP（所有近端 0-2 厘米片段呈阳性，所有 2-4 厘米片段呈阴性），但在单根毛发中未检测到（可能在休止期）。在单根头发的每个阳性 2 厘米片段中检测到的 ZP 量范围为 27 至 63 皮克（平均 43 皮克）。头皮毛发（相当于 110,000 根黑发）中 ZP 的总掺入量约为 4.7 μg，相当于单次 10 mg 剂量的约 0.06%（游离 ZP 为 8.03 mg）。此外，利用基质辅助激光解吸电离质谱法成功实现了头发中掺入的单剂量ZP的直接检测及其成像。建议将这些方法结合起来，为记录催眠药物的暴露提供最高级别的证据。
Little information is available on the amounts of hypnotics incorporated into hair after a single administration and about effective analytical procedures to document cases like drug-facilitated sexual assaults. To obtain basic information, single-hair specimens from a volunteer who took a single dose of 10-mg zolpidem (ZP) were analyzed by a newly established liquid chromatography-tandem mass spectrometry procedure, using a one-pot pulverization extraction method. The detection limit of ZP was 50 fg/2-cm single hair, and ZP in each segment was determined for the single black hair specimens (n = 15). ZP was detectable in 14 hairs (positive for all the proximal 0-2 cm segments, negative for all 2-4 cm segments), but was not detected in a single hair (probably in the telogen stage). The amounts of ZP detected in each positive 2-cm segment of single hair ranged from 27 to 63 pg (average 43 pg). The estimated total incorporation of ZP in the scalp hair (black hair similar to 110,000 strands) was about 4.7 mu g, which corresponds to about 0.06 % of the single 10-mg dose (8.03 mg as free ZP). In addition, the direct detection of single-dose ZP incorporated in hair and its imaging were successfully achieved by matrix-assisted laser desorption ionization-mass spectrometry. It is suggested that a combination of these methodologies will provide the highest-level evidence to document such exposure to a hypnotic drug.