Autoimmune diseases: promising emerging therapies.
Autoimmune diseases: promising emerging therapies.
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自身免疫性疾病：有前途的新兴疗法。
DOI：
10.1038/jid.1995.33
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Vandenbark,AA
中科院分区：
文献类型：
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作者：
Vandenbark,AA
结果与正常对照组(中位刺激指数1.3，范围0.97~1.8)相比，加入瘤周细胞培养上清液的淋巴细胞增殖反应明显增强(P=0.012)，经Mann-Whitney U检验。毛乳头细胞和毛囊间成纤维细胞的培养上清液也有类似的反应。TINS的发生与应答细胞的来源无关，也与使用真皮乳头细胞和应答细胞的自体或同种异体组合无关。在使用来自脱发患者非皮损部位的培养物或来自正常对照组的培养物的实验中，没有明显的增殖效应。皮损周围毛乳头细胞培养上清液中IL-6水平(中位数55 ng/ml，范围6.7~440)和非皮损部位细胞株中IL-6水平显著高于正常毛乳头细胞(中位数水平3.0 ng/ml，范围2.6~8.5)，p=0.037，Mann Whitney U检验。在进行B9检测之前，用多克隆羊抗人IL-6抗血清中和证实了这些上清液中IL-6的生物学活性。用预先与IL-6抗血清孵育1h的上清标本进行淋巴细胞增殖实验，仅有少量的增殖反应下降，但差异不显著(方差分析)，提示IL-6在诱导所观察到的有丝分裂效应方面只起到不大的作用。在正常毛囊中，Mx抗体染色仅局限于内根鞘。仅在斑秃周围组织的毛囊中观察到Mx蛋白染色增加。与正常头皮组织相比，Mx的表达增强主要分布在皮损生发毛囊外根鞘的角化细胞和基质中，真皮乳头中的表达也略有增加。
RESULTSA lymphocyte proliferative response was observed in cultures incorporating supernatants from cultured cells derived from perilesional tissues (median stimulation index 17.5, range 9.8-36), in contrast to normal controls (median stimulation index 1.3, range 0.97-1.8), p= 0.012, Mann-Whitney U test. Similar responses were observed with supernatants derived from both dermal papilla cells and interfollicular fibroblasts. TIns occurred irrespective of the source of responder cells and whether autologous or allogeneic combinations of dermal papilla cells and responder cells were used. No sigrllficant proliferative effect occurred in experiments using media from cultures derived from non-Iesional sites in alopecia arcata patients, or from normal control subjects. Significantly higher levels of IL-6 were detected in superna tants derived from perilesional dermal papilla cells (median level 55 ng/ml, range 6.7-440) and in one cell line from a non lesional site than in those from normal dermal papilla cells (median level 3.0 ng/ml, range 2.6-8.5), p= 0.037, Mann Whitney U test. Biologic activity of the IL-6 in these superna tants was confirmed by neutralization with polyclonal goat anti-human IL-6 antiserum prior to performing the B9 assay. When the lymphocyte proliferation assay was repeated using supernatant samples pre-incubated for 1 h with nentralizing IL-6 antiserum, there was only a small reduction in the proliferative response that was not significant (analysis of variance), suggest ing IL-6 was of only modest importance in inducing the mitogenic effect observed. In normal hair follicles, staining with the Mx antibodies was confined to the inner root sheath. Increased staining for Mx protein was observed within the hair follicles of perilesional alopecia areata tissues only. The distri bution of increased Mx expression was mainly in the keratino cytes of the outer root sheath and matrix of lesional anagen follicles but was mildly increased also in the dermal papilla compared to normal scalp tissue.