A novel classification of tumour budding in colorectal cancer based on the presence of cytoplasmic pseudo-fragments around budding foci
A novel classification of tumour budding in colorectal cancer based on the presence of cytoplasmic pseudo-fragments around budding foci
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DOI：
10.1111/j.1365-2559.2005.02162.x
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发表时间：
2005-07-01
期刊：
影响因子：
6.4
通讯作者：
Jass, JR
中科院分区：
文献类型：
--
作者：
Shinto, E;Mochizuki, H;Jass, JR
目的：肿瘤萌芽是结直肠癌（CRC）的一个不良预后因素。我们已经研究了细胞质片段发生在肿瘤出芽病灶附近的意义。方法和结果：根据出芽程度（10-19和20+）和细胞角蛋白免疫染色鉴定的细胞质片段的存在与否，对73例高级别出芽的crc进行分类（x20物镜内bbb10出芽灶）。在连续切片中，细胞质片段显示为与出芽肿瘤细胞连续性的树突状细胞过程，并被重新命名为伪片段。细胞质伪片段与β -连环蛋白（P = 0.045）和层粘连蛋白-5 - γ - 2 （P < 0.0001）的异常表达相关，而与瘤周淋巴细胞浸润缺失相关（P = 0.0077）。细胞质伪片段与浸润生长方式（P = 0.0014）的相关性强于肿瘤出芽程度（P = 0.014）。芽殖程度与细胞质伪片段无相关性（P = 0.12）。结论：细胞质伪片段可能是一种激活的出芽表型的标志，这种表型与结直肠癌的细胞运动和侵袭性增加有关，与出芽的程度无关。
Aims: Tumour budding is an adverse prognostic factor in colorectal cancer (CRC). We have investigated the significance of cytoplasmic fragments occurring in the immediate vicinity of tumour budding foci.Methods and results: Seventy-three CRCs with high-grade budding (> 10 budding foci in a x 20 objective field) were classified according to extent of budding (10-19 versus 20+ foci) and by the presence or absence of cytoplasmic fragments identified by immunostaining for cytokeratin. In serial sections, cytoplasmic fragments were shown to be dendritic cell processes in continuity with budding tumour cells and were renamed pseudo-fragments. Cytoplasmic pseudo-fragments, but not extent of budding, were associated with aberrant expression of beta-catenin (P = 0.045) and laminin-5 gamma 2 (P < 0.0001), and with absent peritumoral lymphocytic infiltration (P = 0.0077). Cytoplasmic pseudo-fragments had a stronger association with infiltrating growth pattern (P = 0.0014) than extent of tumour budding (P = 0.014). There was no association between extent of budding and cytoplasmic pseudo-fragments (P = 0.12).Conclusions: Cytoplasmic pseudo-fragments may be a marker for an activated budding phenotype that is associated with cell motility and increased invasiveness in CRC and is independent of the extent of budding.