Hierarchical self-assembly of F-actin and cationic lipid complexes: stacked three-layer tubule networks.
Hierarchical self-assembly of F-actin and cationic lipid complexes: stacked three-layer tubule networks.
复制标题
DOI：
10.1126/science.288.5473.2035
复制
发表时间：
2000-06
期刊：
影响因子：
56.9
通讯作者：
G. Wong;Jay X. Tang;Alison J. Lin;Youli Li;P. Janmey;C. Safinya
中科院分区：
文献类型：
--
作者：
G. Wong;Jay X. Tang;Alison J. Lin;Youli Li;P. Janmey;C. Safinya
我们描述了细胞骨架丝状肌动蛋白（F-肌动蛋白）（一种高电荷聚电解质）和阳离子脂质膜的一种独特类型的自发分层自组装。在介观长度尺度上，共聚焦显微镜揭示了带状小管结构，这些结构在宏观尺度（超过 100 微米）上连接形成小管网络。在管内，在 0.5 至 50 纳米长度范围内，X 射线衍射揭示了一种不寻常的结构，由渗透膨胀的复合膜堆叠组成，在简单的两亲系统中没有直接类似物。该复合膜由三层组成，脂质双层夹在两层肌动蛋白之间，让人想起远离平衡状态的多层细菌细胞壁。电子显微镜显示肌动蛋白层由横向锁定的 F-肌动蛋白丝组成，形成各向异性的二维束缚晶体，这似乎是小管形成的起源。
We describe a distinct type of spontaneous hierarchical self-assembly of cytoskeletal filamentous actin (F-actin), a highly charged polyelectrolyte, and cationic lipid membranes. On the mesoscopic length scale, confocal microscopy reveals ribbonlike tubule structures that connect to form a network of tubules on the macroscopic scale (more than 100 micrometers). Within the tubules, on the 0.5- to 50-nanometer length scale, x-ray diffraction reveals an unusual structure consisting of osmotically swollen stacks of composite membranes with no direct analog in simple amphiphilic systems. The composite membrane is composed of three layers, a lipid bilayer sandwiched between two layers of actin, and is reminiscent of multilayered bacterial cell walls that exist far from equilibrium. Electron microscopy reveals that the actin layer consists of laterally locked F-actin filaments forming an anisotropic two-dimensional tethered crystal that appears to be the origin of the tubule formation.