Establishment and characterization of NCC-DDLPS1-C1: a novel patient-derived cell line of dedifferentiated liposarcoma
Establishment and characterization of NCC-DDLPS1-C1: a novel patient-derived cell line of dedifferentiated liposarcoma
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NCC-DDLPS1-C1 的建立和表征：一种新型的患者来源的去分化脂肪肉瘤细胞系
DOI：
10.1007/s13577-020-00436-5
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发表时间：
2020
期刊：
影响因子：
4.3
通讯作者：
Kondo Tadashi
中科院分区：
文献类型：
--
作者：
Tsuchiya Ryuto;Yoshimatsu Yuki;Noguchi Rei;Sei Akane;Takeshita Fumitaka;Sugaya Jun;Fukushima Suguru;Yoshida Akihiko;Ohtori Seiji;Kawai Akira;Kondo Tadashi
去分化脂肪肉瘤（DDLPS）是脂肪肉瘤的四种亚型之一，其特征是12 q13 -15区域扩增，其中包括MDM 2和CDK 4基因。DDLPS具有极高的局部复发率，且化疗和放疗难以治疗，导致预后不良。因此，迫切需要建立一种新的治疗策略来改善DDLPS的预后。虽然患者来源的细胞系是基础研究的重要工具，但公共细胞库中没有DDLPS细胞系。在这里，我们报告了一种新的DDLPS细胞系的建立。利用手术切除的DDLPS患者的肿瘤组织，我们建立了一个细胞系，并将其命名为NCC-DDLPS 1-C1。NCC-DDLPS 1-C1细胞含有12 q13 -15、1 p32和1 q23扩增子，并高度表达MDM 2和CDK 4蛋白。NCC-DDLPS-C1细胞在小鼠中表现出恒定生长、球体形成、侵袭性侵袭和成瘤性。通过筛选药物库，我们确定蛋白酶体抑制剂硼替佐米对NCC-DDLPS 1-C1细胞的增殖具有抑制作用。我们的结论是，NCC-DDLPS 1-C1细胞系可以作为一个有用的工具，DDLPS的基础和临床前研究。
Dedifferentiated liposarcoma (DDLPS) is one of the four subtypes of liposarcomas; it is characterized by the amplification of the 12q13-15 region, which includesMDM2andCDK4genes. DDLPS has an extremely high local recurrence rate and is refractory to chemotherapy and radiation, which leads to poor prognosis. Therefore, a novel therapeutic strategy should be urgently established for improving the prognosis of DDLPS. Although patient-derived cell lines are important tools for basic research, there are no DDLPS cell lines available from public cell banks. Here, we report the establishment of a novel DDLPS cell line. Using the surgically resected tumor tissue from a patient with DDLPS, we established a cell line and named it NCC-DDLPS1-C1. The NCC-DDLPS1-C1 cells contained 12q13-15, 1p32, and 1q23 amplicons and highly expressed MDM2 and CDK4 proteins. NCC-DDLPS-C1 cells exhibited constant growth, spheroid formation, aggressive invasion, and tumorigenesis in mice. By screening a drug library, we identified that the proteasome inhibitor, bortezomib, had inhibitory effects on the proliferation of NCC-DDLPS1-C1 cells. We concluded that the NCC-DDLPS1-C1 cell line may serve as a useful tool for basic and pre-clinical studies of DDLPS.