In vivo imaging of quantum dots encapsulated in phospholipid micelles
In vivo imaging of quantum dots encapsulated in phospholipid micelles
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DOI：
10.1126/science.1077194
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发表时间：
2002-11-29
期刊：
影响因子：
56.9
通讯作者：
Libchaber, A
中科院分区：
文献类型：
--
作者：
Dubertret, B;Skourides, P;Libchaber, A
荧光半导体纳米晶(量子点)有可能给生物成像带来革命性的变化，但由于难以获得生物相容的纳米晶，它们的使用受到了限制。为了解决这个问题，我们将单个纳米晶体包裹在磷脂嵌段共聚胶束中，并展示了体外和体内成像。当与DNA结合时，纳米晶-胶束在体外表现出类似的作用。与特定互补序列杂交的荧光探针。此外，当注射到非洲爪哇胚胎中时，纳米晶胶束是稳定的，无毒(
Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block-copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nanocrystal-micelles acted as in vitro. fluorescent probes to hybridize to specific complementary sequences. Moreover, when injected into Xenopus embryos, the nanocrystal-micelles were stable, nontoxic (