Affinity chromatography: a review of clinical applications.
Affinity chromatography: a review of clinical applications.
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DOI：
10.1093/clinchem/45.5.593
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发表时间：
1999-05
影响因子：
9.3
通讯作者：
D. Hage
中科院分区：
文献类型：
--
作者：
D. Hage
亲和色谱是一种液相色谱，利用类似生物的相互作用来分离和特异性分析样品成分。本综述介绍了亲和色谱的基本原理，并探讨了其在临床样品检测中的应用，重点介绍了基于 HPLC 的方法。这种方法的一些传统应用包括使用硼酸盐、凝集素、蛋白 A 或蛋白 G，以及用于直接定量溶质的免疫亲和支持。详细研究了使用基于抗体的柱进行在线或离线样品提取的新技术，以及将亲和色谱与其他分析方法（例如反相液相色谱、气相色谱和毛细管电泳）结合使用的方法。还描述了间接分析物检测方法，其中使用免疫亲和层析来进行基于流的免疫测定。审查的其他应用包括基于亲和力的手性分离以及使用亲和色谱法研究药物或激素与结合蛋白的相互作用。然后考虑未来可能发展的一些领域，例如串联亲和方法以及使用合成染料、固定金属离子、分子印记或适体作为临床分析物的亲和配体。
Affinity chromatography is a type of liquid chromatography that makes use of biological-like interactions for the separation and specific analysis of sample components. This review describes the basic principles of affinity chromatography and examines its use in the testing of clinical samples, with an emphasis on HPLC-based methods. Some traditional applications of this approach include the use of boronate, lectin, protein A or protein G, and immunoaffinity supports for the direct quantification of solutes. Newer techniques that use antibody-based columns for on- or off-line sample extraction are examined in detail, as are methods that use affinity chromatography in combination with other analytical methods, such as reversed-phase liquid chromatography, gas chromatography, and capillary electrophoresis. Indirect analyte detection methods are also described in which immunoaffinity chromatography is used to perform flow-based immunoassays. Other applications that are reviewed include affinity-based chiral separations and the use of affinity chromatography for the study of drug or hormone interactions with binding proteins. Some areas of possible future developments are then considered, such as tandem affinity methods and the use of synthetic dyes, immobilized metal ions, molecular imprints, or aptamers as affinity ligands for clinical analytes.