Factors involved in the success ofCastanea henryistem cuttings in different cutting mediums and cutting selection periods
Factors involved in the success ofCastanea henryistem cuttings in different cutting mediums and cutting selection periods
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不同扦插介质和选穗期影响锥栗茎插条成功的因素
DOI：
10.1007/s11676-020-01208-5
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发表时间：
2020-09-01
影响因子：
3
通讯作者：
Yuan, Deyi
中科院分区：
文献类型：
--
作者：
Chen, Wangzun;He, Libing;Yuan, Deyi
锥栗（Castaneahenryi）是我国重要的木材和淀粉两用树种。研究了4种扦插基质（纯蛭石、泥炭：河沙= 3：1、泥炭：黑钙土= 1：1和纯黑钙土）和3个扦插时期（3月、5月和7月）对红毛锦鸡儿扦插生根的影响。亨利插条不同扦插时期和扦插基质对扦插生根率有较大影响。henryi的含量为3.35 ~ 77.31%。主成分分析结果表明，扦插时期和扦插基质的最佳组合为半硬枝扦插（5月扦插）+红壤。组织学证据表明，不定根的原始存在的第5-6周，和根原基起始的网站观察维管形成层。不同时期茎解剖结构的观察表明，木质部/半径比为29.90-37.42%，韧皮部纤维环断裂是生根成功的标志。生根指数与培养基性质的关系模型表明，培养基中营养物质含量和孔隙度对愈伤组织的产生有显著影响。pH值对C. henryi根的形成，与皮尔逊相关系数为-0.856和-0.947 5月和7月扦插分别。该方法有助于实现改良C. henryi基因型，从而克服了栗树育种计划中的常见障碍。
Chinquapin (Castanea henryi) is a dual-purpose tree species in China valued for as a source of timber and starch. We investigated the effect of four cutting mediums (pure vermiculite; peat:river sand at 3:1 v/v; peat:krasnozem at 1:1 v/v; and pure krasnozem) and three stem cutting periods (March, May, and July) on rooting performance of C. henryi cuttings. Different cutting periods and cutting mediums greatly influenced the rooting rate of C. henryi, ranging from 3.35 to 77.31%. Principal component analysis indicated that the best combination of cutting period and cutting medium was semi-hardwood cuttings (May cuttings) + krasnozem. Histological evidence indicated that adventitious root initials were present by week 5–6, and that the site of root primordia initiation was observed in the vascular cambium. Stem anatomical structures observed at different periods indicated that a xylem/radius ratio of 29.90–37.42% and a fractured phloem fiber ring are indicative of rooting success. The relational model between rooting index and medium properties indicated that nutrient content and porosity significantly influenced callus production. However, pH strongly affected C. henryi root formation, with the Pearson correlation coefficients for May and July cuttings of − 0.856 and − 0.947, respectively. Our protocol is helpful to achieve mass clone propagation of improved C. henryi genotypes, thus overcoming a common hurdle in chinquapin breeding programs.