GPR39 receptor expression in the mouse brain
GPR39 receptor expression in the mouse brain
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DOI：
10.1097/01.wnr.0000215779.76602.93
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发表时间：
2006-05-29
期刊：
影响因子：
1.7
通讯作者：
Civelli, Olivier
中科院分区：
文献类型：
--
作者：
Jackson, Valerie R.;Nothacker, Hans-Peter;Civelli, Olivier
GPR39是一种孤儿G蛋白偶联受体，最近被确定为生物活性肽肥胖抑制素的受体。肥胖抑制素由胃分泌，是一种抑制食欲的激素。无论是腹腔内给药还是脑室内给药，都会诱导这种活性。已知GPR39在中枢神经系统中表达，但其确切定位尚不清楚。鉴于该系统日益重要，我们决定通过原位杂交研究GPR39 mRNA的表达部位。我们发现杏仁核、海马体和听觉皮层中GPR39 mRNA水平最高，而在其他几个脑区水平较低。令人惊讶的是，我们发现下丘脑中没有GPR39的表达，而下丘脑本应是肥胖抑制素产生厌食作用的部位。
GPR39, an orphan G protein-coupled receptor, has been recently identified as the receptor for the bioactive peptide obestatin. Obestatin is secreted from the stomach and acts as an anti-appetite hormone. This activity is induced whether obestatin is administered intraperitoneally or intracerebroventricularly. GPR39 is known to be expressed in the central nervous system but its precise localization is unknown. In view of the growing importance of this system, we decided to study the sites of GPR39 mRNA expression by in-situ hybridization. We find the highest levels of GPR39 mRNA in the amygdala, the hippocampus, and the auditory cortex and low levels in several other brain regions. Surprisingly, we find no expression of GPR39 in the hypothalamus, expected to be the site of the anorexigenic action of obestatin.