Multiplexed Tissue Tomography.
Multiplexed Tissue Tomography.
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DOI：
10.1007/978-1-0716-1593-5_6
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Lee SS
中科院分区：
文献类型：
--
作者：
Phillips EH;Scholten D;Flor AC;Kron SJ;Lee SS
多路组织断层扫描能够对整个组织或厚组织切片内的标记物进行全面的空间分析。透明剂通常用于使组织透明并促进深部组织成像。目前在各种组织类型中使用许多清除和组织断层摄影的方法。在这里，我们详细介绍了一种称为透明组织断层扫描（T3）的工作流程，它建立在以前的方法基础上，可应用于难以清除的组织，如肿瘤。
Multiplexed tissue tomography enables comprehensive spatial analysis of markers within a whole tissue or thick tissue section. Clearing agents are often used to make tissue transparent and facilitate deep tissue imaging. Many methods of clearing and tissue tomography are currently used in a variety of tissue types. Here we detail a workflow known as Transparent Tissue Tomography (T3), which builds upon previous methods and can be applied to difficult to clear tissues such as tumors.