MiR-144 regulates adipogenesis by mediating formation of C/EBPa-FOXO1 protein complex
MiR-144 regulates adipogenesis by mediating formation of C/EBPa-FOXO1 protein complex
复制标题
MiR-144 通过介导 C/EBPα-FOXO1 蛋白复合物的形成来调节脂肪生成
DOI：
10.1016/j.bbrc.2022.04.093
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发表时间：
2022-05-04
影响因子：
3.1
通讯作者：
Chen, Jie
中科院分区：
文献类型：
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作者：
Lin, Weimin;Wen, Xianyu;Chen, Jie
ceRNA效应是miRNA介导生物活性的重要调控方式，但目前对ceRNA的研究多集中在ncRNA与mRNA的协同作用上，对ceRNA效应调控mRNA相互作用的探讨尚不多见。此外，C/EBPa是最重要的脂肪形成调节因子之一，已被证明与FOXO 1形成蛋白复合物以介导AdipoQ的表达。因此，我们试图探讨ceRNA效应是否介导了C/EBPa和FOXO 1的相互作用，并鉴定其ceRNA效应的关键miRNAs。本研究发现C/EBPa和FOXO 1的ceRNA效应介导了它们的蛋白复合物的形成，进而调节其对AdipoQ的转录作用，从而影响前脂肪细胞的脂肪形成。更重要的是，我们证明了miR-144是介导C/EBPa和FOXO 1的ceRNA效应影响AdipoQ从而调节前脂肪细胞脂肪形成的决定性因素。本研究为miRNA介导编码RNA相互作用调控前脂肪细胞脂肪形成提供了新的补充思路。(c)2022爱思唯尔公司All rights reserved.
CeRNA effect was an important regulation mode of miRNA mediated bio-activities, however, most of the researches of ceRNA were on ncRNAs synergetic with mRNAs, the exploration of ceRNA effect regulated mRNA interaction was still lack of. Besides, C/EBPa was one of the most crucial adipogenic regulators, which has been demonstrated to form a protein complex with FOXO1 to mediate AdipoQ expression. So that, we try to explore whether the ceRNA effect mediated the interaction of C/EBPa and FOXO1, and identified the key miRNAs of their ceRNA effect. In this paper, we found the ceRNA effect of C/EBPa and FOXO1 mediated their protein complex formation, furthermore regulated its transcriptional role for AdipoQ, thereby influencing pre-adipocytes adipogenesis. More importantly, we demonstrated that the miR-144 was the decisive factor that mediated the ceRNA effect of C/EBPa and FOXO1 to influence AdipoQ, thus regulated pre-adipocytes adipogenesis. This research will provide a new supplementary idea of the miRNA role in mediating coding RNA interaction that regulates pre-adipocyte adipogenesis. (c) 2022 Elsevier Inc. All rights reserved.