Genomic and epigenetic alterations in diploid gynogenetic hybrid fish
Genomic and epigenetic alterations in diploid gynogenetic hybrid fish
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二倍体雌核杂交鱼的基因组和表观遗传改变
DOI：
10.1016/j.aquaculture.2019.734383
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发表时间：
2019
期刊：
影响因子：
4.5
通讯作者：
Liu Shaojun
中科院分区：
文献类型：
--
作者：
Wu Chang;Chen Qian;Huang Xu;Hu Fangzhou;Zhu Shurun;Luo Linlin;Gong Dingbin;Gong Kaijun;Zhao Rurong;Zhang Chun;Qin Qinbo;Tao Min;Liu Shaojun
雌核发生作为一种有效的基因组操纵技术，在水产养殖相关研究中得到了相当广泛的应用。然而，关于异源二倍体杂交鱼与杂交体基因组产生单倍体配子的雌核发生的知识很少。本研究主要分析了具有改良性状的雌交杂交种的基因组和表观遗传变化。成熟的卵子的第四代(BTF4 2 n = 48)allodiploid混合血统的冲鼻子鲷(BSB, 2 n = 48,Megalobrama amblycephala,♀) × topmouth鲌属(TC, 2 n = 48岁鲌属alburnus,♂)被紫外线照射过的精子从鲤鱼跃出,激活(锦鲤,2 n = 100,鲤属carpiohaematopterus,♂),冷休克翻了一倍。DNA含量分析和形态观察证实所有子代均为雌性二倍体（GBTF4, 2n = 48），表明btf4卵具有杂交基因组。微卫星基因组调查结果显示，gbtf4基因组与BTF4基因组同源率为87.72%，与BTF4基因组同源率为12.28%，遗传率为0.03%。另一方面，甲基化敏感扩增多态性（MSAP）结果显示，gbtf4基因组的DNA甲基化水平（38.61%）低于btf4基因组（40.99%）。此外，MSAP分析还显示，在随机选择的588个CCGG位点上，与btf4相比，gbtf4的甲基化模式改变了30.1%。这些结果丰富了我们对异体二倍体杂交鱼杂交与雌核发生结合以及雌核发生后基因组和表观遗传变化的认识，为鱼类遗传育种提供了新的见解。
Gynogenesis, as an effective technology to manipulate genome, is used fairly widely in aquaculture-related research. However, the knowledge regarding gynogenesis in allodiploid hybrid fish with the hybrid genome producing haploid gametes is sparse. This study focused on the analyses on the genomic and epigenetic alterations of the gynogenetic hybrids with improved trait. The mature eggs of the fourth generation (BTF4, 2n = 48) of allodiploid hybrid lineage of blunt snout bream (BSB, 2n = 48,Megalobrama amblycephala, ♀) × topmouth culter (TC, 2n = 48,Culter alburnus, ♂) were activated by UV-irradiated sperm from koi carp (Koi, 2n = 100,Cyprinus carpiohaematopterus, ♂) and doubled by cold shock. The analyses of the DNA content and morphological observation confirmed that all of the progenies were gynogenetic diploids (GBTF4, 2n = 48), suggesting that the eggs of BTF4possessed the hybrid genome. A genomic survey resulted from microsatellite indicated that 87.72% of the GBTF4genome was the same as that of BTF4, 12.28% of the GBTF4genome exhibited polymorphism compared with that of BTF4, and 0.03% of the GBTF4genome was inherited from Koi. On the other hand, the results of methylation sensitive amplification polymorphism (MSAP) showed that the DNA methylation level (38.61%) in the GBTF4genome was lower than that (40.99%) in the BTF4genome. In addition, MSAP profiling also showed 30.1% methylation pattern changes in GBTF4compared with BTF4at 588 randomly selected CCGG sites. These results enriched our knowledge regarding the combination of hybridization and gynogenesis and the genomic and epigenetic alterations in allodiploid hybrid fish following process of gynogenesis, providing new insights into fish genetic breeding.