Molecular epidemiology of hepatitis B virus in the United Republic of Tanzania
Molecular epidemiology of hepatitis B virus in the United Republic of Tanzania
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DOI：
10.1002/jmv.20659
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发表时间：
2006-08-01
影响因子：
12.7
通讯作者：
Mizokami, Masashi
中科院分区：
文献类型：
--
作者：
Hasegawa, Izumi;Tanaka, Yasuhito;Mizokami, Masashi
在坦桑尼亚联合共和国，457名自愿献血者参加了B型肝炎病毒（HBV）血清学筛查; 4.8%（22/457）携带HBsAg，其中13.6%（3/22）为HBeAg阳性。HBeAg阴性携带者的平均年龄为31岁。HBV DNA检出率为81.8%（18/22），平均水平为3.67（± 1.77）log copies/ml。90.9%（20/22）为A型，18/20为Aa亚型（亚洲/非洲）。在18个菌株中分析了基本核心启动子、前核心和部分核心核苷酸序列; T1809/T1812（“Kozak”序列）和A/T1888（突变信号）变体分别被鉴定为100%和78%。对其中一个坦桑尼亚菌株的全基因组测序显示没有重组。总之，在坦桑尼亚的一般人群中，HBV血清阳性率很高，HBV/Aa感染占主导地位。早期HBeAg血清转换和病毒载量下降的趋势应在病例对照研究中进一步证实。
In the United Republic of Tanzania, 457 voluntary blood donors were enrolled in hepatitis B virus (HBV) serological screening; 4.8% (22/457) carried HBsAg, 13.6% (3/22) of whom were HBeAg-positive. The mean age among HBeAg-negative carriers was 31 years. HBV DNA was detectable in 81.8% (18/22), the mean level was 3.67 (+/- 1.77) log copies/ml. Genotype A was determined in 90.9% (20/22) and 18/20 were classified into subgenotype Aa (Asia/Africa). The basal core promoter, precore and partial core nucleotide sequences were analyzed in the 18 strains; T1809/T1812 ("Kozak" sequence) and A/T1888 (encapsidation signal) variants were identified in 100% and 78%, respectively. The complete genome sequencing for one of the Tanzanian strains revealed no recombination. In conclusion, HBV seroprevalence is high among general population in Tanzania, and the HBV/Aa-infection is predominant. The indicated tendency to early HBeAg seroconversion and declining of the viral load should be confirmed further in case-control studies.