Genomics of Three New Bacteriophages Useful in the Biocontrol of Salmonella.
Genomics of Three New Bacteriophages Useful in the Biocontrol of Salmonella.
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DOI：
10.3389/fmicb.2016.00545
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发表时间：
2016
影响因子：
5.2
通讯作者：
Llagostera M
中科院分区：
文献类型：
--
作者：
Bardina C;Colom J;Spricigo DA;Otero J;Sánchez-Osuna M;Cortés P;Llagostera M
非伤寒沙门氏菌是世界范围内食源性疾病的主要病原体。广泛的抗生素耐药性对人类健康产生了不利影响，并鼓励人们寻找替代抗菌剂。噬菌体治疗的进展突出了它们在控制广泛的食源性病原体方面的应用。使用噬菌体作为抗菌剂的一个要求是表征它们的基因组。在这项工作中，对能够感染多种沙门氏菌菌株的三种新的有毒沙门氏菌特异性噬菌体（UAB_Phi20， UAB_Phi78和UAB_Phi87）进行了全基因组测序和分子分析。对UAB_Phi20、UAB_Phi78和UAB_Phi87噬菌体基因组的序列分析未发现已知的毒力相关基因和抗生素抗性基因，以及潜在的免疫反应性食物过敏原。UAB_Phi20基因组包含41809个碱基对和80个开放阅读框（orf）；其中24个都有指定的功能。基因组序列显示UAB_Phi20与沙门氏菌噬菌体P22及足病毒科其他P22样病毒属（包括ST64T和ST104）具有高度同源性。UAB_Phi78的DNA长度为44110 bp，其中直接末端重复序列（DTR）为179 bp，预测了58个假定的orf， 20个指定了功能。该噬菌体不仅与SP6，而且与感染大肠杆菌的K1-5、K1E和K1F噬菌体高度相似，因此被归为足病毒科的SP6样病毒属。UAB_Phi87基因组序列为87,669 bp，末端直接重复数为608 bp；虽然确定了148个orf，但其中只有29个可被赋予假定的功能。序列比较表明，UAB_Phi87与大肠杆菌噬菌体Felix O1和wV8在基因含量和功能组织上具有较高的相似性。大末端酶亚基的系统发育分析证实了它们的包装策略和分组到不同的噬菌体属类型。所有这些研究都是必要的，以开发和使用有效的鸡尾酒与商业应用的噬菌体治疗沙门氏菌。
Non-typhoid Salmonella is the principal pathogen related to food-borne diseases throughout the world. Widespread antibiotic resistance has adversely affected human health and has encouraged the search for alternative antimicrobial agents. The advances in bacteriophage therapy highlight their use in controlling a broad spectrum of food-borne pathogens. One requirement for the use of bacteriophages as antibacterials is the characterization of their genomes. In this work, complete genome sequencing and molecular analyses were carried out for three new virulent Salmonella-specific bacteriophages (UAB_Phi20, UAB_Phi78, and UAB_Phi87) able to infect a broad range of Salmonella strains. Sequence analysis of the genomes of UAB_Phi20, UAB_Phi78, and UAB_Phi87 bacteriophages did not evidence the presence of known virulence-associated and antibiotic resistance genes, and potential immunoreactive food allergens. The UAB_Phi20 genome comprised 41,809 base pairs with 80 open reading frames (ORFs); 24 of them with assigned function. Genome sequence showed a high homology of UAB_Phi20 with Salmonella bacteriophage P22 and other P22likeviruses genus of the Podoviridae family, including ST64T and ST104. The DNA of UAB_Phi78 contained 44,110 bp including direct terminal repeats (DTR) of 179 bp and 58 putative ORFs were predicted and 20 were assigned function. This bacteriophage was assigned to the SP6likeviruses genus of the Podoviridae family based on its high similarity not only with SP6 but also with the K1-5, K1E, and K1F bacteriophages, all of which infect Escherichia coli. The UAB_Phi87 genome sequence consisted of 87,669 bp with terminal direct repeats of 608 bp; although 148 ORFs were identified, putative functions could be assigned to only 29 of them. Sequence comparisons revealed the mosaic structure of UAB_Phi87 and its high similarity with bacteriophages Felix O1 and wV8 of E. coli with respect to genetic content and functional organization. Phylogenetic analysis of large terminase subunits confirms their packaging strategies and grouping to the different phage genus type. All these studies are necessary for the development and the use of an efficient cocktail with commercial applications in bacteriophage therapy against Salmonella.