A simple and rapid method for the analysis of fish histamine by paper electrophoresis
A simple and rapid method for the analysis of fish histamine by paper electrophoresis
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DOI：
10.1111/j.1444-2906.2006.01232.x
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发表时间：
2006-08
影响因子：
1.9
通讯作者：
Minoru Satô;Zhihua Tao;K. Shiozaki;T. Nakano;Toshiyasu Yamaguchi;Takehiko Yokoyama;N. Kan-no;E. Nagahisa
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文献类型：
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作者：
Minoru Satô;Zhihua Tao;K. Shiozaki;T. Nakano;Toshiyasu Yamaguchi;Takehiko Yokoyama;N. Kan-no;E. Nagahisa
所描述的鱼类和海鲜中组胺测定的分析方法包括样品提取、吸附到纸盘上、将纸盘应用到电泳纸上、电泳仅10分钟、干燥和用保利试剂显色。组胺可以被令人满意地检测到，并与组氨酸、肌肽和其他保利试剂阳性化合物完全分离。该方法不需要昂贵的仪器和任何繁琐的预处理来消除其他咪唑化合物（例如组氨酸或肌肽）的潜在干扰。该方法可用于同时检测多个鱼类和海鲜样品中的组胺，这些样品中的组胺含量低至 15 p.p.m.。组胺（1.5 毫克/100 克）。
The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly’s reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).