Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.
Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance.
复制标题
粘膜纤毛运输装置的构建和应用以研究粘膜纤毛清除。
DOI：
10.1007/978-1-0716-3507-0_17
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Ostrowski,LawrenceE
中科院分区：
文献类型：
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作者：
Sears,PatrickR;Ostrowski,LawrenceE
气道上皮细胞的高度分化的气液界面培养物产生并分泌粘液，并且具有在顶端流体中搏动的丰富纤毛。在培养物中，这种纤毛跳动不是很好地协调或发生在小的焦点区域，因此产生的粘膜纤毛运输（MCT）仅在短距离内是线性的。我们提出了一种方法，诱导纤毛细胞在培养过程中对齐生长。细胞沿着一个确定的圆形轨道的轴排列，从而产生一个协调良好的旋转运输，这是有效的线性纤毛细胞的长度尺度。这些改良的插入物-被称为粘膜纤毛转运装置（MCTD）-制备简单，并且在高百分比的培养物中可重复地产生结果，证明了完全循环转运（CCT）。
Well-differentiated air-liquid interface cultures of airway epithelial cells produce and secrete mucus and have abundant cilia that beat in the apical fluid. In cultures, this ciliary beating is not well coordinated or occurs in small focal areas so the resulting mucociliary transport (MCT) is only linear over short distances. We present a method which induces ciliated cells in cultures to align during growth. The cells align along the axis of a defined circular track, thus producing a well-coordinated rotational transport which is effectively linear on length scales of ciliated cells. These modified inserts – referred to as mucociliary transport devices (MCTDs) – are simple to prepare and result reproducibly in a high percentage of cultures demonstrating complete circular transport (CCT).