Peptide Amphiphile Mediated Co-assembly for Nanoplasmonic Sensing.
Peptide Amphiphile Mediated Co-assembly for Nanoplasmonic Sensing.
复制标题
用于纳米等离子体传感的肽两亲物介导的共组装。
DOI：
10.1002/anie.202214394
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Jokerst,JesseV
中科院分区：
文献类型：
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作者：
Jin,Zhicheng;Li,Yi;Li,Ke;Zhou,Jiajing;Yeung,Justin;Ling,Chuxuan;Yim,Wonjun;He,Tengyu;Cheng,Yong;Xu,Ming;Creyer,MatthewN;Chang,Yu-Ci;Fajtová,Pavla;Retout,Maurice;Qi,Baiyan;Li,Shuzhou;O'Donoghue,AnthonyJ;Jokerst,JesseV
Aromatic interactions are commonly involved in the assembly of naturally occurring building blocks, and these interactions can be replicated in an artificial setting to produce functional materials. Here we describe a colorimetric biosensor using co‐assembly experiments with plasmonic gold and surfactant‐like peptides (SLPs) spanning a wide range of aromatic residues, polar stretches, and interfacial affinities. The SLPs programmed in DDD−(ZZ)x−FFPC self‐assemble into higher‐order structures in response to a protease and subsequently modulate the colloidal dispersity of gold leading to a colorimetric readout. Results show the strong aggregation propensity of the FFPC tail without polar DDD head. The SLPs were specific to the target protease, i.e., Mpro, a biomarker for SARS‐CoV‐2. This system is a simple and visual tool that senses Mproin phosphate buffer, exhaled breath condensate, and saliva with detection limits of 15.7, 20.8, and 26.1 nM, respectively. These results may have value in designing other protease testing methods.
