A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells
A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells
复制标题
DOI：
10.1016/j.canlet.2004.03.001
复制
发表时间：
2004-08-30
期刊：
影响因子：
9.7
通讯作者：
Matsuse, T
中科院分区：
文献类型：
--
作者：
Ishii, T;Fujishiro, M;Matsuse, T
谷胱甘肽S转移酶P1(GSTP1)是一种异源酶，被认为与化疗耐药有关，尽管有报道称GSTP1基因启动子的胞嘧啶-鸟嘌呤岛甲基化抑制了GSTP1基因的甲基化，但该启动子甲基化程度不高，且GSTP1蛋白在肺癌中高表达。当我们将甲基化的寡核苷酸转导到A549肺腺癌细胞后，GSTP1启动子的甲基化和GSTP1表达的减少，细胞存活率下降，但对顺铂的耐药性并没有明显改变。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Glutathione S-transferase P1 (GSTP1) belongs to xenobiotic enzymes, and is supposed to contribute to chemoresistance, Though it was reported that GSTP1 gene is suppressed by cytosine-guanine (CpG) island methylation of its promoter, this promoter is not strongly methylated and GSTP1 protein is highly expressed in lung cancer.We intended to induce methylation of GSTP1 CpG island by using a methylated sense oligonucleotide complementary to this region. When we transduced the methylated oligonucleotides to A549 lung adenocarcinoma cells, methylation of the GSTP1 promoter and reduction of GSTP1 expression was induced, cell viability was reduced; however, chemoresistance against cisplatin has not clearly changed. (C) 2004 Elsevier Ireland Ltd. All rights reserved.