Characterisation of clinical isolates of oxacillin-susceptible mecA-positive Staphylococcus aureus in China from 2009 to 2014.
Characterisation of clinical isolates of oxacillin-susceptible mecA-positive Staphylococcus aureus in China from 2009 to 2014.
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DOI：
10.1016/j.jgar.2017.05.009
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发表时间：
2017-12
影响因子：
4.6
通讯作者：
Yunjia Song;Lanqing Cui;Y. Lv;Yun Li;F. Xue
中科院分区：
文献类型：
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作者：
Yunjia Song;Lanqing Cui;Y. Lv;Yun Li;F. Xue
目的了解2009年7月至2014年6月中国临床奥西林敏感菌阳性金黄色葡萄球菌（OS-MRSA）的流行情况及分子特征。方法非重复标本共2068份。从21家医院采集金黄色葡萄球菌。采用琼脂稀释法进行药敏试验。筛选所有OS-MRSA菌株是否存在genesmecA、mecCandnucas和pton - valentine leukocidin基因（pvl）。采用葡萄球菌盒式染色体（SCCmec）分型、葡萄球菌蛋白A （spa）分型和多位点序列分型（MLST）对分离物进行基因型分析。结果共34S。金黄色葡萄球菌对肟西林敏感[最低抑制浓度(MIC)≤2 mg/L]。所有OS-MRSA分离株均对头孢西丁耐药，多数对除β-内酰胺类外的其他抗生素多重耐药。在34株OS-MRSA分离株中，检测到9株和3株sccmecv型，与MLST合并，ST338/59-t437-SCCmecV（47%; 16/34）为优势克隆。另外，17株（50%）携带pvlgene。结论中国OS-MRSA分离株最常见的克隆为ST338-t437-SCCmecV。除大环内酯类、克林霉素和氯霉素外，大多数OS-MRSA菌株对大多数抗菌药物均敏感。
ObjectivesThe aim of this study was to investigate the prevalence and molecular characteristics of clinical oxacillin-susceptiblemecA-positiveStaphylococcus aureus(OS-MRSA) isolates in China from July 2009 to June 2014.MethodsA total of 2068 non-duplicateS. aureusisolates were collected from 21 hospitals. Antimicrobial susceptibility testing was performed by the agar dilution method. All OS-MRSA strains were screened for the presence of the genesmecA,mecCandnucas well as the Panton–Valentine leukocidin gene (pvl). Staphylococcal cassette chromosomemec(SCCmec) typing, staphylococcal protein A (spa) typing and multilocus sequence typing (MLST) were performed to analyse the isolate genotypes.ResultsA total of 34S. aureusisolates weremecA-positive but were susceptible to oxacillin [minimum inhibitory concentration (MIC) ≤ 2 mg/L]. All OS-MRSA isolates were resistant to cefoxitin and most were also multiresistant to other antibiotics besides β-lactams. Among the 34 OS-MRSA isolates, ninespaand three SCCmectypes were detected and, combined with MLST, ST338/59-t437-SCCmecV (47%; 16/34) was the predominant clone. In addition, 17 strains (50%) carried thepvlgene.ConclusionsThe most frequent clone of OS-MRSA isolates in China was ST338-t437-SCCmecV. Most of the OS-MRSA isolates were susceptible to the majority of antibacterial agents except macrolides, clindamycin and chloramphenicol.