Bacillus volatiles adversely affect the physiology and ultra-structure of Ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt.
Bacillus volatiles adversely affect the physiology and ultra-structure of Ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt.
复制标题
芽孢杆菌挥发物对青枯菌的生理和超微结构产生不利影响，并诱导烟草对青枯病的系统抗性
DOI：
10.1038/srep40481
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发表时间：
2017-01-16
影响因子：
4.6
通讯作者：
Gao X
中科院分区：
文献类型：
--
作者：
Tahir HA;Gu Q;Wu H;Niu Y;Huo R;Gao X
各种细菌产生的挥发性有机化合物（VOC）具有促进植物生长和控制植物病原体的巨大潜力。六种最有效的拮抗芽孢杆菌属。本研究中使用了青枯菌 (Rsc) TBBS1（烟草青枯病的病原体）。解淀粉芽孢杆菌FZB42和脂肪芽孢杆菌LSSC22对Rsc的抑制作用最强。使用气相色谱-质谱分析鉴定了 FZB42 产生的 13 种 VOC 和 LSSC22 产生的 10 种 VOC。苯甲醛、1,2-苯并异噻唑-3(2 H)-酮和1,3-丁二烯显着抑制病原体的集落大小、细胞活力和运动性，并对趋化性产生负面影响。透射和扫描电子显微镜显示 Rsc 细胞的形态和超微结构发生严重变化。此外，VOCs改变了PhcA（一种全球毒力调节因子）、III型分泌系统（T3SS）、IV型分泌系统（T4SS）、胞外多糖和趋化性相关基因的转录表达水平，这些基因是致病性的主要贡献者，从而减少了枯萎病的发生。挥发性有机化合物显着上调了与抗枯萎病和病原体防御相关的基因的表达。 EDS1 和 NPR1 的过度表达表明 SA 途径参与诱导系统耐药。我们的研究结果为抗菌挥发性有机化合物作为对抗青枯病的生物防治工具的潜力提供了新的见解。
Volatile organic compounds (VOCs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. Six of the most effective antagonistic Bacillus spp. were used in this study against Ralstonia solanacearum (Rsc) TBBS1, the causal agent of bacterial wilt disease in tobacco. Bacillus amyloliquefaciens FZB42 and Bacillus artrophaeus LSSC22 had the strongest inhibitory effect against Rsc. Thirteen VOCs produced by FZB42 and 10 by LSSC22 were identified using gas chromatography-mass spectrometry analysis. Benzaldehyde, 1,2-benzisothiazol-3(2 H)-one and 1,3-butadiene significantly inhibited the colony size, cell viability, and motility of pathogens and negatively influenced chemotaxis. Transmission and scanning electron microscopy revealed severe morphological and ultra-structural changes in cells of Rsc. Furthermore, VOCs altered the transcriptional expression level of PhcA (a global virulence regulator), type III secretion system (T3SS), type IV secretion system (T4SS), extracellular polysaccharides and chemotaxis-related genes, which are major contributors to pathogenicity, resulting in decreased wilt disease. The VOCs significantly up-regulated the expression of genes related to wilt resistance and pathogen defense. Over-expression of EDS1 and NPR1 suggest the involvement of SA pathway in induction of systemic resistance. Our findings provide new insights regarding the potential of antibacterial VOCs as a biocontrol tool against bacterial wilt diseases.