A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment
A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment
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DOI：
10.1073/pnas.2136683100
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发表时间：
2003-12-23
影响因子：
11.1
通讯作者：
Gold, L
中科院分区：
文献类型：
--
作者：
Daniels, DA;Chen, H;Gold, L
将分子库靶向复杂系统而不是生物化学纯实体是一种可以鉴定生物学感兴趣的蛋白质的可行方法。我们已经探测了由单层肿瘤细胞呈递的抗原与适体库相互作用的能力。胶质母细胞瘤衍生的细胞系U251被用作通过使用单链DNA文库进行指数富集的配体系统进化的靶标。我们分离出特异性相互作用的寡核苷酸，并使用生化策略来鉴定其中一种适体的蛋白质靶点。在这里，我们的特点的DNA适体，GBI-10，与生腱蛋白-C，在肿瘤基质中发现的细胞外蛋白质的相互作用。生腱蛋白-C被认为参与胚胎发生和肿瘤发生途径。通过指数富集的配体的系统进化似乎是复杂系统内生物学感兴趣的目标的先验识别的成功策略。
The targeting of molecular repertoires to complex systems rather than biochemically pure entities is an accessible approach that can identify proteins of biological interest. We have probed antigens presented by a monolayer of tumor cells for their ability to interact with a pool of aptamers. A glioblastoma-derived cell line, U251, was used as the target for systematic evolution of ligands by exponential enrichment by using a single-stranded DNA library. We isolated specifically interacting oligonucleotides, and biochemical strategies were used to identify the protein target for one of the aptamers. Here we characterize the interaction of the DNA aptamer, GBI-10, with tenascin-C, an extracellular protein found in the tumor matrix. Tenascin-C is believed to be involved in both embryogenesis and oncogenesis pathways. Systematic evolution of ligands by exponential enrichment appears to be a successful strategy for the a priori identification of targets of biological interest within complex systems.