Discrimination of repetitive sequences polymorphism in Secale cereale by genomic in situ hybridization-banding.
Discrimination of repetitive sequences polymorphism in Secale cereale by genomic in situ hybridization-banding.
复制标题
DOI：
10.1111/j.1744-7909.2008.00644.x
复制
发表时间：
2008-04
影响因子：
11.4
通讯作者：
Jianping Zhou;Zujun Yang;Guangrong Li;Cheng Liu;Z. Ren
中科院分区：
文献类型：
--
作者：
Jianping Zhou;Zujun Yang;Guangrong Li;Cheng Liu;Z. Ren
利用基因组原位杂交显带（GISH-banding）技术，对中国黑麦（Secale cereale L.）使我们能够可视化单个黑麦染色体，并创建一个通用的参考核型的S。cereale染色体1 R至7 R。本研究中采用的GISH显带方法能够区分S。小麦（Triticum aestivum L.）背景，包括小黑麦、小麦-黑麦附加系和易位系。此外，S.谷类亚种Afghanicum与中国黑麦cv.而瓦氏黑麦的GISH带型与荆州黑麦不同。这表明，GISH分带可用于黑麦属种或亚种的进化多态性研究。此外，本文还讨论了GISH显带技术的产生及其在富含腺嘌呤-胸腺嘧啶异染色质研究中的应用。
Genomic in situ hybridization banding (GISH-banding), a technique slightly modified from conventional GISH, was used to probe the Chinese native rye (Secale cereale L.) DNA, and enabled us to visualize the individual rye chromosomes and create a universal reference karyotype of the S. cereale chromosome 1R to 7R. The GISH-banding approach used in the present study was able to discriminate S. cereale chromosomes or segments in the wheat (Triticum aestivum L.) background, including the Triticale, wheat-rye addition and translocation lines. Moreover, the GISH-banding pattern of S. cereale subsp. Afghanicum chromosomes was consistent with that of Chinese native rye cv. Jingzhou rye; whereas the GISH-banding pattern of Secale vavilovii was different from that of S. cereale, indicating that GISH-banding can be used to study evolutionary polymorphism in species or subspecies of Secale. In addition, the production and application of GISH-banding to the study of adenine-thymine-riched heterochromatin is discussed.