PICRIC ACID METHODS GREATLY OVERESTIMATE SERUM CREATININE IN MICE - MORE ACCURATE RESULTS WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
PICRIC ACID METHODS GREATLY OVERESTIMATE SERUM CREATININE IN MICE - MORE ACCURATE RESULTS WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/0003-2697(85)90118-6
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发表时间：
1985-01-01
影响因子：
2.9
通讯作者：
IRWIN, RL
中科院分区：
文献类型：
--
作者：
MEYER, MH;MEYER, RA;IRWIN, RL
用高效液相色谱法测定人、大鼠和小鼠的血和尿肌酐水平。将这些结果与标准比色、动力学和自动分析技术进行的碱性苦味酸肌酐分析进行了比较。对于人的血清和尿液，用高效液相技术测得的结果与5种碱性苦味酸法中的4种符合得很好。对于黑鼠或白鼠，血清肌酐浓度为8.7+-。高效液相色谱法测定0.4µm，而44.9.+-。用最低碱性苦味酸法测定1.9µm。小鼠尿肌酐浓度为3.24±-。HPLC0.19 mm，4.59+-。0.39 mm采用最接近的碱性苦味酸盐法。用高效液相色谱法分析大鼠血清肌酐浓度约为自动分析仪测定值的一半。小鼠和大鼠的样品似乎含有非特异性显色的物质，因此干扰了碱性苦味酸法测定肌酐。虽然碱性苦味酸法测定肌酐对人体样本是合适的，但要准确测定啮齿类动物的肌酐，则需要使用高效液相色谱法。采用动力法和高效液相色谱法测定小鼠尿和血浆肌酐，并与尿液和血浆菊粉进行比较，以确定小鼠尿和血浆肌酐排泄分数。利用动力学方法，肌酐在43.+-处被清除。菊粉得率为3%。用高效液相色谱法，肌酐在170。+-处被清除。菊粉得率为11%。
The creatinine levels of blood and urine from humans, rats and mice were measured by high-performance liquid chromatography [HPLC]. These were compared to the alkaline picrate analysis of creatinine performed by standard colorimetric, kinetic and AutoAnalyzer techniques. For human serum and urine the values obtained using the HPLC technique gave good agreement with 4 out of 5 alkaline picrate techniques. For black or white mice, the serum creatinine concentrations was 8.7 .+-. 0.4 .mu.M by HPLC but 44.9 .+-. 1.9 .mu.M by the lowest alkaline picrate method. Mouse urine creatinine concentrations were 3.24 .+-. 0.19 mM by HPLC and 4.59 .+-. 0.39 mM by the nearest alkaline picrate method. Rat serum creatinine concentrations analyzed by HPLC were about half the values obtained by AutoAnalyzer. Mouse and rat samples seemed to have substances which gave nonspecific color and thus interfered with the analysis of creatinine by the alkaline picrate methods. While the alkaline picrate analysis of creatinine was adequate for human samples, it was necessary to use HPLC to accurately measure rodent creatinine. The fractional excretion of creatinine was determined by measuring creatinine in mouse urine and plasma by both the kinetic and HPLC methods and comparing these values to urine and plasma inulin. Using the kinetic method, creatinine was cleared at 43 .+-. 3% of the rate of inulin. Using the HPLC method, creatinine was cleared at 170 .+-. 11% of the rate of inulin.