Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence
Insect-Specific Viruses Detected in Laboratory Mosquito Colonies and Their Potential Implications for Experiments Evaluating Arbovirus Vector Competence
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DOI：
10.4269/ajtmh.14-0330
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发表时间：
2015-02-01
影响因子：
3.3
通讯作者：
Popov, Vsevolod L.
中科院分区：
文献类型：
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作者：
Bolling, Bethany G.;Vasilakis, Nikos;Popov, Vsevolod L.
最近，在野外采集的蚊子中检测和鉴定昆虫特有病毒的工作大幅增加。有证据表明，这些病毒在自然界中无处不在，许多病毒是通过在蚊子种群中垂直传播而维持的。一些研究表明，昆虫特有病毒的存在可能会抑制超级感染虫媒病毒的复制，从而改变蚊子宿主的媒介能力。因此，我们对实验室的蚊子群体进行了昆虫特有病毒的筛查。将蚊群匀浆后接种于白纹伊蚊C6/36细胞。对感染细胞进行电子显微镜检查，并对RNA提取液进行深度测序。电子显微镜图像显示，在我们的三个实验室蚊子群体中存在三种不同的病毒。讨论了这些发现对媒介能力研究的潜在意义。
Recently, there has been a dramatic increase in the detection and characterization of insect-specific viruses in field-collected mosquitoes. Evidence suggests that these viruses are ubiquitous in nature and that many are maintained by vertical transmission in mosquito populations. Some studies suggest that the presence of insect-specific viruses may inhibit replication of a super-infecting arbovirus, thus altering vector competence of the mosquito host. Accordingly, we screened our laboratory mosquito colonies for insect-specific viruses. Pools of colony mosquitoes were homogenized and inoculated into cultures of Aedes albopictus (C6/36) cells. The infected cells were examined by electron microscopy and deep sequencing was performed on RNA extracts. Electron micrograph images indicated the presence of three different viruses in three of our laboratory mosquito colonies. Potential implications of these findings for vector competence studies are discussed.