Identification of Burkholderia multivorans ATCC 17616 genes induced in soil environment by in vivo expression technology
Identification of Burkholderia multivorans ATCC 17616 genes induced in soil environment by in vivo expression technology
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利用体内表达技术鉴定土壤环境中诱导的多食伯克霍尔德氏菌 ATCC 17616 基因
DOI：
10.1111/j.1462-2920.2010.02227.x
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发表时间：
2010
影响因子：
5.1
通讯作者：
and M. Tsuda
中科院分区：
文献类型：
--
作者：
Nishiyama;E.;Y. Ohtsubo;Y. Nagata;and M. Tsuda
多重伯克霍尔德氏菌ATCC 17616最初是从土壤样品中分离出来的，它携带三条染色体。为了鉴定可能对土壤定殖具有适应性意义的性状，以无启动子和随机排列的dapBandlacZ基因为报告基因，构建了atcc 17616的活体表达技术体系，并应用该体系对atcc 17616在灭菌土壤中诱导的基因组座位进行了鉴定。通过对DAPB-LacZ插入克隆文库的筛选，分离到713个克隆，其中基因组的插入位点可能在土壤中转录，而不是在实验室培养基中转录。以基因组DNA为模板，通过高通量测序确定了基因组中的所有插入位点，随后的分析得到了总共116个基因组座位的可靠列表，即在土壤环境中诱导的多重芽孢杆菌ATCC 17616基因座。这些116mls携带着从各种物质获得能量的基因，以及细胞包膜完整性和生态位适应的基因。这些基因座的分布偏向于第二条染色体，这表明该复制子作为增强该有机体在自然环境中生存的适应性特征的来源具有重要意义。
Burkholderia multivoransATCC 17616 was originally isolated from a soil sample, and it carries three chromosomes. To identify traits of likely adaptive significance for colonization of soil, anin vivoexpression technology system for ATCC 17616 was constructed using the promoterless and tandemly arrangeddapBandlacZgenes as the reporters, and this system was applied to identify the genomic loci of ATCC 17616 that were induced in sterilized soil. Our screening of a library consisting ofdapB‐lacZ‐inserted clones resulted in the isolation of 713 clones in which the insertion sites of genome were putatively transcribed in the soil but not in laboratory media. All insertion sites in the genome were determined by high‐throughput sequencing using genomic DNA as the templates, and subsequent analysis led to a reliable list of a total of 116 genomic loci as theB.multivoransATCC 17616loci induced in asoil environment (mls). These 116mlscarried the genes for energy acquisition from various substances, as well as genes for cell‐envelope integrity and the niche adaptation. The distribution of these loci was biased to the second chromosome, suggesting the importance of this replicon as a source of adaptive traits enhancing survival of this organism in natural environments.