Core–Shell Microfibers via Bioorthogonal Layer-by-Layer Assembly
Core–Shell Microfibers via Bioorthogonal Layer-by-Layer Assembly
复制标题
通过生物正交逐层组装的核壳超细纤维
DOI：
10.1021/acsmacrolett.0c00515
复制
发表时间：
2020
影响因子：
7.015
通讯作者：
Jia, X.
中科院分区：
文献类型：
--
作者：
Ravikrishnan, A.;Zhang, H.;Fox, J. M.;Jia, X.
介绍了一种用于生物医学应用的核-壳结构微纤维的制备方法。采用静电纺丝法制备纤维支架，然后利用反式环辛烯（TCO）与四嗪（Tz）之间的快速生物正交反应，通过共价层层沉积法制备纤维支架。对静电纺丝聚（ε-己内酯）（PCL）支架进行表面改性以安装四嗪基团。将支架反复浸没在TCO改性的透明质酸（HA-TCO）和四嗪改性的透明质酸（HA-Tz）的水溶液中，导致交联的HA凝胶在各个微纤维周围的受控生长。使用TCO缀合的RGD肽将整合素结合基序共价连接到微纤维的表面。支架促进了原代猪声带成纤维细胞的附着和生长，而没有显著诱导肌成纤维细胞表型。用转化生长因子β（TGF-β）刺激适度增强成纤维细胞活化，并且使用Y27632抑制Rho/ROCK信号通路进一步降低成肌纤维细胞标志物的表达。生物正交组装的支架与一个刚性的PCL核心和一个软HA壳可能会发现应用程序作为治疗性植入物治疗声带瘢痕。
A new technique is described for the construction of core–shell microfibers for biomedical applications. Fibrous scaffolds were fabricated by electrospinning, followed by covalent layer-by-layer deposition based on the rapid bioorthogonal reaction betweens-tetrazines (Tz) andtrans-cyclooctenes (TCOs). Electrospun poly(ε-caprolactone) (PCL) scaffolds were subjected to surface modifications to install tetrazine groups. The scaffolds were iteratively submerged in aqueous solutions of TCO-modified hyaluronic acid (HA-TCO) and tetrazine-modified hyaluronic acid (HA-Tz), resulting in the controlled growth of a cross-linked HA gel around individual microfibers. Integrin-binding motifs were covalently attached to the surface of the microfibers using TCO-conjugated RGD peptide. The scaffolds fostered the attachment and growth of primary porcine vocal fold fibroblasts without a significant induction of the myofibroblast phenotype. Stimulation with transforming growth factor beta (TGF-β) moderately enhanced fibroblast activation, and inhibition of the Rho/ROCK signaling pathway using Y27632 further decreased the expression of myofibroblastic markers. The bioorthogonally assembled scaffolds with a stiff PCL core and a soft HA shell may find application as therapeutic implants for the treatment of vocal fold scarring.