Effect of heparin oligomer chain length on the activation of valvular interstitial cells.
Effect of heparin oligomer chain length on the activation of valvular interstitial cells.
复制标题
肝素寡聚物链长度对瓣膜间质细胞活化的影响。
DOI：
10.1021/bm100211k
复制
发表时间：
2010
影响因子：
6.2
通讯作者：
Anseth,KristiS
中科院分区：
文献类型：
--
作者：
Pedron,Sara;Kasko,AndreaM;Peinado,Carmen;Anseth,KristiS
结缔组织重塑的一个关键事件涉及成纤维细胞向肌成纤维细胞的转化，α-平滑肌肌动蛋白（α-SMA）的表达也揭示了这一点。然而，这种转变的失调可能会导致纤维化，由于细胞外基质沉积过多而导致组织过度生长和硬化，这一过程与心脏瓣膜疾病和许多其他疾病有关。疾病治疗和再生策略都将受益于可促进或抑制这种转化的生物活性因子的受控递送和呈现策略。在这方面，肝素复合多种生长因子的能力为此目的提供了广泛的可能性。在此，探讨了肝素链长度和结构对瓣膜间质细胞（VIC）表型表达的影响。取自猪肠粘膜的肝素经肝素酶解聚，分级分离得到不同大小的寡糖。与其他糖类和全长肝素相比，用八糖和十糖培养的 VIC 表现出更高的 a-SMA 表达。没有观察到 VIC 因培养基中存在全长肝素而激活。
A key event in connective tissue remodeling involves the transformation of fibroblasts to myofibroblasts, also revealed by expression of α-smooth muscle actin (α-SMA). However, misregulation of this transition can lead to fibrosis, an overgrowth and hardening of tissue due to excess extracellular matrix deposition, a process that is linked to heart valve disease and many others. Both disease treatment and regenerative strategies would benefit from strategies for the controlled delivery and presentation of bioactive factors that can promote or suppress this transformation. In this regard, the ability of heparin to complex a plethora of growth factors offers a broad range of possibilities for this purpose. Here, the effects of heparin chain length and structure on valvular interstitial cell (VIC) phenotypic expression were explored. Heparin from porcine intestinal mucosa was depolymerized with heparinase and fractionated to obtain oligosaccharides of different sizes. VICs cultured with octasaccharides and decasaccharides exhibited higher expression of a-SMA when compared to other saccharides and full-length heparin. No activation of VICs was observed in response to full-length heparin presence in media.