Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups.
Synthesis of Oligodeoxynucleotides Containing Electrophilic Groups.
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DOI：
10.1021/acs.orglett.6b01878
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发表时间：
2016-08-05
期刊：
影响因子：
5.2
通讯作者：
Fang S
中科院分区：
文献类型：
--
作者：
Lin X;Chen J;Shahsavari S;Green N;Goyal D;Fang S
以1，3-二硫-2-甲氧基羰基(Dmoc)为保护基团和连接剂，在非亲核氧化条件下实现了寡核苷酸(ODN)的脱保护和裂解。亲核敏感硫酸酯和α-氯乙酰基被方便地结合到寡核苷酸序列中。该技术对亲电合成ODN具有普遍的实用价值。
Using 1,3-dithian-2-yl-methoxycarbonyl (Dmoc) as protecting groups and linker for oligodeoxynucleotide (ODN) synthesis, deprotection and cleavage are achieved under non-nucleophilic oxidative conditions. The nucleophile-sensitive thioester and α-chloroacetyl groups are conveniently incorporated into ODN sequences. The technology could be universally useful for electrophilic ODN synthesis.