An assessment of olfaction and responses to novelty in three strains of mice.
An assessment of olfaction and responses to novelty in three strains of mice.
复制标题
对三种小鼠品系的嗅觉和对新奇事物的反应进行评估。
DOI：
10.1016/j.bbr.2009.01.024
复制
发表时间：
2009
影响因子：
2.7
通讯作者：
Johnson,AlexanderW
中科院分区：
文献类型：
--
作者：
Smith,DaniR;Burruss,DanielR;Johnson,AlexanderW
在啮齿类动物中，最初暴露于新的刺激或环境通常会诱发探索。经过长时间的接触，探索的水平下降。最近，我们开发了一种基于气味的新奇检测范式，广泛筛选功能，如嗅觉感知，嗅觉驱动的探索和习惯化，以及新奇偏好和记忆。这种模式的优势在于它利用了老鼠天生的嗅觉能力。在这里，我们研究了三种小鼠品系C57 BL/6（C57），129/SvImJ（129），以及这两种品系的杂交（F1杂交），所有这些都是通常用于产生转基因小鼠的。在该任务的第一阶段，允许小鼠探索测试环境以适应它。随后是样品阶段，其中将两个相同的气味立方体引入测试环境，并允许小鼠探索两个气味立方体。最后，在测试阶段，其中一种气味线索被一个含有不同的新气味的立方体所取代，并再次允许小鼠探索。通常，小鼠会表达对新刺激的偏好，或者在这种情况下，新气味立方体。我们还单独评估了简单的气味检测。我们的研究结果表明，与C57小鼠相比，129和F1小鼠表现出探索和气味驱动的新奇偏好水平降低。
In rodents, the initial exposure to a novel stimulus or environment typically induces exploration. After prolonged exposure the level of exploration decreases. Recently we developed an odor-based novelty detection paradigm that broadly screens for functions such as olfactory perception, olfactory driven exploration and habituation, and novelty preference and memory. The advantage of such a paradigm is that it exploits the innate olfactory abilities of mice. Here we studied three strains of mice C57BL/6 (C57), 129/SvImJ (129), and a hybrid cross of these two strains (F1 hybrids), all of which are commonly used in the generation of genetically modified mice. In the first phase of this task mice are permitted to explore the test environment in order to habituate to it. This is followed by a sample phase in which two identical odor cubes are introduced to the test environment and the mice are allowed to explore both odor cubes. Finally during the test phase one of the odor cues is replaced with a cube that contains a different novel odor, and the mice are again allowed to explore. Typically, mice will express a preference for the novel stimulus, or in this case the novel odor cube. We also separately assessed simple odor detection. Our results show that compared to the C57 mice, 129 and F1 mice showed reduced levels of exploration and odor driven novelty preference.