ADENOVIRUS DNA-REPLICATION INVITRO - ORIGIN AND DIRECTION OF DAUGHTER STRAND SYNTHESIS
ADENOVIRUS DNA-REPLICATION INVITRO - ORIGIN AND DIRECTION OF DAUGHTER STRAND SYNTHESIS
复制标题
DOI：
10.1016/0022-2836(79)90524-2
复制
发表时间：
1979-01-01
影响因子：
5.6
通讯作者：
KELLY, TJ
中科院分区：
文献类型：
--
作者：
CHALLBERG, MD;KELLY, TJ
从腺病毒感染的[人宫颈癌HeLa]细胞能够复制外源性添加的腺病毒DNA在体外的可溶性酶系统的特点。当从纯化的腺病毒病毒粒子分离的DNA-蛋白质复合物作为模板加入时，观察到最大的DNA合成。在这些条件下，DNA复制在模板的任一末端或其附近开始。然后子链合成以5“至3”方向进行，置换相同极性的亲本链。在腺病毒基因组的常规图谱上，r子链从右到左合成，l子链从左到右合成。这一过程与体内腺病毒DNA复制过程相同。当将去蛋白腺病毒DNA加入体外系统时，观察到的有限DNA合成似乎是由于修复样反应。合成可以在模板内的许多位点开始，合成产物主要由与模板DNA链共价连接的短DNA链组成。
A soluble enzyme system from adenovirus-infected [human cervical carcinoma HeLa] cells capable of replicating exogenously added adenovirus DNA in vitro was characterized. Maximal DNA synthesis is observed when DNA-protein complex, isolated from purified adenovirus virions, is added to as template. Under these conditions DNA replication starts at or near either end of the template. Daughter strand synthesis then proceeds in the 5'' to 3'' direction displacing the parental strand of the same polarity. The r daughter strand is synthesized from right to left on the conventional map of the adenovirus genome, and the l daughter strand is synthesized from left to right. This course of events is the same as that which occurs during adenovirus DNA replication in vivo. When deproteinized adenovirus DNA is added to the in vitro system, the limited DNA synthesis that is observed appears to be due to a repair-like reaction. Synthesis can begin at many sites within the template, and the synthetic product consists largely of short DNA chains that are covalently linked to template DNA strands.