Adaptive evolution of the African and Indonesian coelacanths to deep-sea environments.
Adaptive evolution of the African and Indonesian coelacanths to deep-sea environments.
复制标题
DOI：
10.1016/s0378-1119(00)00474-1
复制
发表时间：
2000-12
期刊：
影响因子：
3.5
通讯作者：
Shozo Yokoyama;Takashi Tada
中科院分区：
文献类型：
--
作者：
Shozo Yokoyama;Takashi Tada
我们对印度尼西亚腔棘鱼的视紫红质（RH 1）和进化上密切相关的RH 2基因进行了PCR扩增和测序，现在被称为Latimeria menadoensis。当构建RH 1和RH 2编码序列，在培养的细胞中表达，并用11-顺式-视黄醛重建时，所得视色素的最大吸收波长（λmax）分别为485和479 nm。这些λ max值与非洲腔棘鱼（Latimeria chalumanus）的λ max值相同，表明印度尼西亚腔棘鱼也能探测到狭窄的颜色范围。统计分析表明，腔棘鱼对深海的适应早在2亿年前就开始了。
We have PCR amplified and sequenced the rhodopsin (RH1) and evolutionarily closely related RH2 genes of the Indonesian coelacanth, now referred to as Latimeria menadoensis. When the RH1 and RH2 coding sequences are constructed, expressed in cultured cells, and reconstituted with 11-cis-retinal, the resulting visual pigments have wavelengths of maximal absorption (λmax) of 485 and 479 nm, respectively. These λmaxvalues are identical to those of the African coelacanth, Latimeria chalumnae, showing that the Indonesian coelacanths also detect a narrow range of color. Statistical analyses show that the adaptation of the coelacanths toward the deep-sea started as early as 200 million years ago.