Effects of ovarian cancer G protein coupled receptor 1 on the proliferation, migration, and adhesion of human ovarian cancer cells
Effects of ovarian cancer G protein coupled receptor 1 on the proliferation, migration, and adhesion of human ovarian cancer cells
复制标题
卵巢癌G蛋白偶联受体1对人卵巢癌细胞增殖、迁移和粘附的影响。
DOI：
10.3760/cma.j.issn.0366-6999.2011.09.010
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发表时间：
2011-05-05
影响因子：
6.1
通讯作者：
Zhang Long
中科院分区：
文献类型：
--
作者：
Ren Juan;Zhang Long
背景OGR 1是一种G蛋白偶联受体（GPCR）和质子传感器。我们前期的研究发现OGR 1对前列腺癌的转移有抑制作用。为探讨OGR 1基因在卵巢癌生物学行为中的作用，本研究通过将OGR 1基因转染到内源性低表达的HEY细胞中，观察OGR 1基因对HEY细胞的影响。在不同的测定中比较OGR 1过表达的细胞和载体转染的细胞。Western blotting检测OGR 1的表达水平。通过MU测定和细胞倍增时间测定来测定细胞增殖。采用细胞迁移实验（transwell实验）和细胞粘附实验测定细胞的迁移和粘附能力。结果OGR 1高表达组细胞增殖能力明显降低（P
Background OGR1 was found as a G-protein coupled receptor (GPCR) and proton sensor. Our previous studies have found that OGR1 has inhibitory effect on the metastasis of prostate cancer. In order to investigate the roles of OGR1 gene in the biological activities of ovarian cancer, we studied the OGR1 effects on ovarian cancer cells, HEY cells.Methods OGR1 gene was transfected into HEY cell, in which endogenous expression is low. OGR1-overxepressed cells and vector-transfected cells were compared in different assays. Western blotting was employed to confirm the high expression level of OGR1. Cell proliferation was determined by MU assay and cell doubling time assay. Cell migration assay (transwell assay) and cell adhesion assay were performed to determine the migration and adhesion potential of cells. Student's t test was employed for statistical analysis.Results Proliferation of OGR1-overexpressed cells was significantly reduced (P