Association of pulse wave velocity with single nucleotide polymorphisms related to parathyroid hormone
Association of pulse wave velocity with single nucleotide polymorphisms related to parathyroid hormone
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脉搏波速度与甲状旁腺激素相关单核苷酸多态性的关联
DOI：
10.1080/08037051.2018.1445961
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发表时间：
2018
期刊：
影响因子：
1.8
通讯作者：
Li Yan
中科院分区：
文献类型：
--
作者：
Cheng Yi-Bang;Guo Qian-Hui;Zhang Dong-Yan;Wang Ying;Huang Qi-Fang;Sheng Chang-Sheng;Wang Ji-Guang;Staessen Jan A.;Li Yan
摘要目的：探讨颈动脉-股动脉脉搏波传导速度（cfPWV）与血清甲状旁腺激素（PTH）的关系。我们在同一队列中研究了cfPWV、臂踝（baPWV）和心臂（hbPWV）脉搏波速度（PWV）是否与rs6127099（CYP 24 A1）和rs 4074995（RGS 14）相关。先前发表的全基因组关联研究表明，T（rs6127099）或G（rs 4074995）等位基因的每一个额外拷贝分别与血清PTH升高7%或3%相关。研究方法：在1601例未经治疗的中国患者（平均年龄51.0岁，51.9%为女性）中，我们通过眼压测量法（SphygmoCor）测量cfPWV，通过联合眼压测量法和体积描记法（VP-2000 PWV/ABI分析仪）测量baPWV和hbPWV，通过免疫测定法测量血清PTH，并通过SNapShot方法测量基因型。结果：cfPWV、baPWV和hbPWV平均为7.9、14.6和5.5m/s，血清PTH平均为65.7pg/mL。基因型频率处于Hardy-Weinberg平衡，rs6127099的基因型频率分别为41.7%（AA）、44.9%（AT）和13.4%（TT），rs 4074995的基因型频率分别为70.7%（GG）、26.9%（GA）和2.3%（AA）。调整性别、年龄、体重指数、心率和季节后，rs6127099的次要等位基因（T）每增加一个拷贝，HbPWV就降低0.05 m/s（p = 0.042）。在对157名年龄小于50岁的血压正常的受试者进行类似调整后的分析中，T等位基因每增加一个拷贝，cfPWV就增加0.32 m/s（p = 0.004）。敏感性分析另外解释了总-HDL血清胆固醇比、血糖、肾小球滤过率和24小时收缩压是一致的。PWV与遗传变异的其他关联没有达到显著性。结论：随着rs6127099 T等位基因数量的增加，动脉僵硬度（以PWV为例）在所有肌性动脉（hbPWV）参与者中较低，但在弹性动脉（cfPWV）中血压正常的年轻参与者中较高。
Abstract Objective: Carotid-femoral pulse wave velocity (cfPWV) was associated with serum parathyroid hormone (PTH) in untreated Chinese. We investigated in the same cohort whether cfPWV, brachial-ankle (baPWV) and heart-brachial (hbPWV) pulse wave velocity (PWV) were associated with rs6127099 (CYP24A1) and rs4074995 (RGS14). A previously published genome-wide association study demonstrated that each additional copy of the T (rs6127099) or G (rs4074995) allele was associated with a 7% or 3% higher serum PTH, respectively. Methods: In 1601 untreated Chinese patients (mean age, 51.0 years; 51.9% women), we measured cfPWV by tonometry (SphygmoCor) and baPWV and hbPWV by combined oscillometry and plethysmography (VP-2000 PWV/ABI analyser), serum PTH by an immunoassay, and genotypes by the SNapShot method. Results: cfPWV, baPWV and hbPWV averaged 7.9, 14.6 and 5.5 m/s and serum PTH 65.7 pg/mL. Genotype frequencies were in Hardy-Weinberg equilibrium, amounting to 41.7% (AA), 44.9% (AT) and 13.4% (TT) for rs6127099 and to 70.7% (GG), 26.9% (GA) and 2.3% (AA) for rs4074995. With adjustments applied for sex, age, body mass index, heart rate and season, hbPWV was 0.05 m/s (p = .042) lower with each additional copy of the minor allele (T) of rs6127099. In similarly adjusted analyses of 157 normotensive participants younger than 50 years, cfPWV was 0.32 m/s (p = .004) higher per additional copy of the T allele. Sensitivity analyses additionally accounting for the total-to-HDL serum cholesterol ratio, plasma glucose, glomerular filtration rate and 24 h systolic blood pressure were consistent. No other association of PWV with the genetic variants reached significance. Conclusions: With an increasing number of rs6127099 T alleles, arterial stiffness, as exemplified by PWV, was lower in all participants in a muscular artery (hbPWV), but higher in young normotensive participants in an elastic artery (cfPWV).