Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.
Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots.
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DOI：
10.1016/j.jip.2014.05.006
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发表时间：
2014-07
影响因子：
3.4
通讯作者：
J. Razinger;M. Lutz;H. Schroers;M. Palmisano;Christian Wohler;G. Urek;J. Grunder
中科院分区：
文献类型：
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作者：
J. Razinger;M. Lutz;H. Schroers;M. Palmisano;Christian Wohler;G. Urek;J. Grunder
在一系列室内和温室试验中，研究了一种潜在的花椰菜根腐病的生物防治策略，即接种多种真菌。除昆虫病原真菌外，还对具有高根际能力的真菌和具有腐养菌在土壤中生存能力的真菌进行了试验。在实验中评估了以下真菌种类：atroviride木霉，T。koningiopsis, T。甘氏菌，球孢白僵菌，金龟子甲基绿僵菌，M。brunneumandClonostachys以上。采用一种市售的以碳硫丹为基础的杀虫剂作为阳性对照。此外，还有两个商业产品，一个基于b。以球孢菌（Naturalis）和苏云金芽孢杆菌（Delfin）为对照生物防治剂。目的是(i)评估选定的真菌分离株对根delia的致病性，（ii）评估真菌分离株的根际能力，重点是原始接种物在生长根上的持久性，（iii）评估可能的植物内生组织定植，（iv）评估添加接种物对植物生长的潜在刺激作用。在室外和温室实验中证实了所测真菌对根腐菌的显著致病性。所有被试真菌都能在花椰菜根面上存活。更重要的是，添加的真菌是在接种点外彻底洗净的根上发现的。这为我们提供了证据，证明我们测试的真菌可以通过伸长的根转移或生长。一些真菌除了在根面上定植外，还在植物的根或茎组织内发现，从而表现出内生特征。讨论了真菌生态学作为合理选择生物防治剂标准的重要性。
A potentialDelia radicumbiological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. In addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. The following fungal species were evaluated in the experiments:Trichoderma atroviride,T. koningiopsis,T. gamsii,Beauveria bassiana,Metharhizium anisopliae,M. brunneumandClonostachys solani. A commercial carbosulfan-based insecticide was used as a positive control. Additionally, two commercial products, one based onB. bassiana(Naturalis) and one onBacillus thuringiensis(Delfin) were used as reference biocontrol agents. The aims were (i) to assess the pathogenicity of the selected fungal isolates toDelia radicum, (ii) to evaluate the fungal isolates’ rhizosphere competence, with the emphasis on the persistence of the original inoculum on the growing roots, (iii) to assess possible endophytic plant tissue colonization, and (iv) to evaluate potential plant growth stimulating effects of the added inoculi. Significant pathogenicity of tested fungi againstDelia radicumwas confirmed inin vitroand glasshouse experiments. All tested fungi persisted on cauliflower rhizoplane. More importantly, the added fungi were found on thoroughly washed roots outside the original point of inoculation. This provided us with evidence that our tested fungi could be transferred via or grow with the elongating roots. In addition to colonizing the rhizoplane, some fungi were found inside the plant root or stem tissue, thus exhibiting endophytic characteristics. The importance of fungal ecology as a criterion in appropriate biological control agent selection is discussed.