Insect tolerance of transgenic Populus nigra plants transformed with Bacillus thuringiensis toxin gene.
Insect tolerance of transgenic Populus nigra plants transformed with Bacillus thuringiensis toxin gene.
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用苏云金芽孢杆菌毒素基因转化的转基因黑杨植物的昆虫耐受性。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
J. Yan
中科院分区：
文献类型：
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作者：
Y. Tian;T. Li;K. Mang;Y. Han;L. Li;X. Wang;M. Lu;L. Dai;J. Yan
利用含有NPT和35S-omega-B.t嵌合基因的双元载体，将根癌农杆菌LBA4404转化黑杨的叶片和茎段。毒素--编号对19株卡那霉素抗性再生植株进行了DNA杂交分析，其中10株为转基因植株。抗虫性试验表明，转基因植株对两种鳞翅目害虫--粉虱Lymantria disparar L.和烟粉虱Apocheimia cinerarius Erschoff均有毒杀作用。根据聚合酶链式反应产物的Southern杂交结果和对其生长和抗虫性的聚类分析，筛选出3株独立的转基因植株，并在苗圃地进行繁殖。这项实地测试目前正在进行中。
Leaves and stem segments of Populus nigra were transformed with A. tumefaciens LBA4404 harboring a binary vector containing chimeric genes of NPT and 35S-omega-B.t. toxin-Nos. Nineteen regenerated kanamycin resistant plants were analyzed by DNA hybridization, out of which 10 were shown to be the candidates of transgenic plants. Insect tolerance tests showed that the transgenic plants were toxic to two lepidopteran pests, Lymantria dispar L. and Apocheimia cinerarius Erschoff. Based on the results of Southern blot of PCR products and the cluster analysis of their growth and insect resistance, three independent transgenic plants were selected, which were then propagated in the nursery field. This field test is currently in progress.