Macroporous Hydrogel Scaffolds and Their Characterization By Optical Coherence Tomography
Macroporous Hydrogel Scaffolds and Their Characterization By Optical Coherence Tomography
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DOI：
10.1089/ten.tec.2010.0072
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Chen, Yu
中科院分区：
文献类型：
--
作者：
Chen, Chao-Wei;Betz, Martha W.;Chen, Yu
提出了一种简单的致孔剂浸出法制备大孔环缩醛水凝胶细胞支架。应用光学相干断层扫描（OCT）对支架结构进行无损成像和定量表征。高分辨率OCT显示了三维的工程化组织支架的微观结构。它还使后续的图像处理，以定量研究几个关键的大孔支架的形态设计参数，包括体积孔隙率，孔互连性，和孔径。采用了两种图像处理算法：三维标记用于评估互连性，腐蚀用于评估孔径。对具有不同设计参数的支架进行成像、表征和比较。OCT成像和图像处理成功地区分了由不同配方制成的支架的体积孔隙率、互连性和孔径。通过与OCT共同配准的荧光显微镜证实细胞活力及其在支架上的扩散。
A simple porogen-leaching method to fabricate macroporous cyclic acetal hydrogel cell scaffolds is presented. Optical coherence tomography (OCT) was applied for nondestructive imaging and quantitative characterization of the scaffold structures. High-resolution OCT reveals the microstructures of the engineered tissue scaffolds in three dimensions. It also enables subsequent image processing to investigate quantitatively several key morphological design parameters for macroporous scaffolds, including the volume porosity, pore interconnectivity, and pore size. Two image-processing algorithms were adapted: three-dimensional labeling was applied to assess the interconnectivity, and erosion was applied to assess the pore size. Scaffolds with different design parameters were imaged, characterized, and compared. OCT imaging and image processing successfully discriminated scaffolds made from different formulations in terms of volume porosity, interconnectivity, and pore size. The cell viability and their spread across the scaffolds were confirmed by the fluorescence microscopy co-registered with OCT.