Ultrastructural characterization of SARS coronavirus
Ultrastructural characterization of SARS coronavirus
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DOI：
10.3201/eid1002.030913
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发表时间：
2004-02-01
影响因子：
11.8
通讯作者：
Zaki, SR
中科院分区：
文献类型：
--
作者：
Goldsmith, CS;Tatti, KM;Zaki, SR
严重急性呼吸系统综合症（SARS）首次被描述是在2002-2003年与人类死亡和人际疾病传播相关的严重肺炎全球暴发期间。病原最初是通过病毒分离物的薄层电子显微镜检查确定为冠状病毒。病毒粒子呈球形，平均直径为78 nm，由一个螺旋状的核衣壳组成，核衣壳位于一个具有表面突起的包膜内。我们发现，感染sars相关冠状病毒导致不同的超微结构特征：双膜囊泡、核衣壳内含物和细胞质的大颗粒区域。超微结构免疫金和原位杂交检测结果显示，这三种结构和冠状病毒颗粒对病毒蛋白和RNA均呈阳性。此外，对SARS患者的细支气管灌洗液标本进行超微结构检查，发现大量冠状病毒感染的细胞，其特征与感染培养细胞相似。电子显微镜研究对于确定SARS爆发的病原和指导随后的实验室和流行病学调查至关重要。
Severe acute respiratory syndrome (SARS) was first described during a 2002-2003 global outbreak of severe pneumonia associated with human deaths and person-to-person disease transmission. The etiologic agent was initially identified as a coronavirus by thin-section electron microscopic examination of a virus isolate. Virions were spherical, 78 nm in mean diameter, and composed of a helical nucleocapsid within an envelope with surface projections. We show that infection with the SARS-associated coronavirus resulted in distinct ultrastructural features: double-membrane vesicles, nucleocapsid inclusions, and large granular areas of cytoplasm. These three structures and the coronavirus particles were shown to be positive for viral proteins and RNA by using ultrastructural immunogold and in situ hybridization assays. In addition, ultrastructural examination of a bronchiolar lavage specimen from a SARS patient showed numerous coronavirus-infected cells with features similar to those in infected culture cells. Electron microscopic studies were critical in identifying the etiologic agent of the SARS outbreak and in guiding subsequent laboratory and epidemiologic investigations.