Tea Tree Oil Prevents Mastitis-Associated Inflammation in Lipopolysaccharide-Stimulated Bovine Mammary Epithelial Cells
Tea Tree Oil Prevents Mastitis-Associated Inflammation in Lipopolysaccharide-Stimulated Bovine Mammary Epithelial Cells
复制标题
茶树油可预防脂多糖刺激的牛乳腺上皮细胞中与乳腺炎相关的炎症
DOI：
10.3389/fvets.2020.00496
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发表时间：
2020-08-07
影响因子：
3.2
通讯作者：
Yang, Zhangping
中科院分区：
文献类型：
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作者：
Chen, Zhi;Zhang, Yi;Yang, Zhangping
本研究的主要目的是探讨茶树油（TTO）对脂多糖（LPS）诱导的奶牛乳腺炎模型的影响。该乳腺炎模型用于确定对TTO和LPS的细胞反应对细胞毒性、mRNA丰度和细胞因子产生的影响。高通量测序用于选择候选基因，然后对这些基因进行功能评价。在第一个实验中，浓度为200 μg/mL的LPS降低细胞增殖，诱导凋亡，并上调肿瘤坏死因子-α（TNF-α）、白细胞介素6（IL-6）和信号转导和转录激活因子1（STAT 1）的蛋白浓度。TTO的加入导致细胞凋亡减少沿着核因子κ B、丝裂原活化蛋白激酶4（MAPK 4）和半胱天冬酶-3的蛋白浓度下调。在第二个实验中，用LPS攻击的BMEC共有1，270个差异表达基因，其中787个上调，483个下调。差异表达基因包括TNF-α、IL 6、STAT 1和MAPK 4。总体而言，结果显示TTO（至少在体外）对LPS诱导的乳腺炎具有保护作用。应进行进一步的体内研究，以确定使用TTO预防和治疗乳腺炎以及改善牛奶质量的策略。
The main purpose of this study was to explore the effect of tea tree oil (TTO) on lipopolysaccharide (LPS)-induced mastitis model using isolated bovine mammary epithelial cells (BMEC). This mastitis model was used to determine cellular responses to TTO and LPS on cellular cytotoxicity, mRNA abundance and cytokine production. High-throughput sequencing was used to select candidate genes, followed by functional evaluation of those genes. In the first experiment, LPS at a concentration of 200 μg/mL reduced cell proliferation, induced apoptosis and upregulated protein concentrations of tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and signal transducer and activator of transcription 1 (STAT1). Addition of TTO led to reduced cellular apoptosis along with downregulated protein concentrations of nuclear factor kappa B, mitogen-activated protein kinase 4 (MAPK4) and caspase-3. In the second experiment, BMEC challenged with LPS had a total of 1,270 differentially expressed genes of which 787 were upregulated and 483 were downregulated. Differentially expressed genes includedTNF-α,IL6, STAT1, andMAPK4. Overall, results showed that TTO (at leastin vitro) has a protective effect against LPS-induced mastitis. Furtherin vivoresearch should be performed to determine strategies for using TTO for prevention and treatment of mastitis and improvement of milk quality.