Influence of postruminal supplementation of methionine and lysine, isoleucine, or all three amino acids on intake and chewing behavior, ruminal fermentation, and milk and milk component production.
Influence of postruminal supplementation of methionine and lysine, isoleucine, or all three amino acids on intake and chewing behavior, ruminal fermentation, and milk and milk component production.
复制标题
瘤胃后补充蛋氨酸和赖氨酸、异亮氨酸或所有三种氨基酸对摄入和咀嚼行为、瘤胃发酵以及乳和乳成分生产的影响。
DOI：
10.2527/1999.77102781x
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发表时间：
1999
影响因子：
3.3
通讯作者：
H. Suzuki
中科院分区：
文献类型：
--
作者：
P. H. Robinson;W. Chalupa;C. Sniffen;W. Julien;H. Sato;T. Fujieda;K. Watanabe;H. Suzuki
四个经产荷斯坦奶牛饲喂基础日粮平衡与康奈尔净蛋白质和碳水化合物系统（CNCPS）。配制的饲料共同限制了蛋氨酸、赖氨酸和异亮氨酸的肠道可吸收供应。奶牛补充没有氨基酸（对照）;赖氨酸和蛋氨酸在瘤胃保护的形式;通过皱胃输注异亮氨酸;或赖氨酸，蛋氨酸，异亮氨酸在4x 4拉丁方安排的治疗与28天的时间。所有处理组奶牛的性能均低于预期，原因是基础日粮的高纤维水平可能导致DM摄入量低。这种高纤维水平可能是饲喂所有日粮的奶牛每日咀嚼时间长的原因，这与高瘤胃pH值一致。DM及其组分的摄入量不受任何治疗的影响。当奶牛饲喂补充有瘤胃保护的赖氨酸和蛋氨酸的饮食时，乳蛋白百分比往往较高;然而，牛奶、牛奶脂肪和牛奶乳糖的产量不受任何治疗的影响。奶牛往往有较高的牛奶乳糖比例，往往产生更多的牛奶和牛奶乳糖时，它们的真胃注入单独的异亮氨酸。然而，当奶牛补充所有三种氨基酸时，产奶量和组成与饲喂未补充饮食的奶牛没有差异。使用CNCPS评价饲喂未补充饲料的奶牛的性能表明，这些奶牛可能受到赖氨酸、异亮氨酸和甲硫氨酸（除代谢蛋白外）的肠道可吸收供应的限制。采用替代模型Shield对未补充饲料进行的评价表明，饲喂未补充饲料的奶牛可能受到赖氨酸、异亮氨酸和精氨酸的肠道可吸收供应的限制。结果表明，增强递送可吸收的异亮氨酸可刺激乳糖合成。
Four multiparous Holstein cows were fed a basal diet balanced with the Cornell Net Protein and Carbohydrate System (CNCPS). Diets were formulated to be co-limiting in intestinally absorbable supplies of methionine, lysine, and isoleucine. Cows were supplemented with no amino acids (control); lysine and methionine in a ruminally protected form; isoleucine by abomasal infusion; or lysine, methionine, and isoleucine in a 4x4 Latin square arrangement of treatments with 28-d periods. Performance of cows on all treatments was lower than expected due to low intake of DM that could have been caused by the high fiber level of the basal diet. This high fiber level was likely responsible for the high daily chewing times for cows fed all diets, which was consistent with the high ruminal pH values. Intake of DM and its components were not influenced by any treatment. Milk protein percentage tended to be higher when cows were fed diets supplemented with ruminally protected lysine and methionine; however, production of milk, milk fat, and milk lactose were not affected by any treatment. Cows tended to have a higher milk lactose proportion and tended to produce more milk and milk lactose when they were abomasally infused with isoleucine alone. However, when cows were supplemented with all three amino acids, milk production and composition did not differ from that of cows fed the unsupplemented diet. Use of the CNCPS to evaluate the performance of the cows fed the unsupplemented diet suggested that these cows may have been colimited by intestinally absorbable supplies of lysine, isoleucine, and methionine in addition to metabolizable protein. Evaluation of the unsupplemented diet with an alternate model, Shield, suggested that cows fed the unsupplemented diet may have been colimited by intestinally absorbable supplies of lysine, isoleucine, and arginine. Results suggest that enhanced delivery of intestinally absorbable isoleucine may stimulate milk lactose synthesis.