Hyaluronidase in Clinical Isolates of Propionibacterium acnes.
Hyaluronidase in Clinical Isolates of Propionibacterium acnes.
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DOI：
10.1155/2015/218918
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Patel R
中科院分区：
文献类型：
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作者：
Tyner H;Patel R
目标。我们试图描述透明质酸酶基因的流行和透明质酸酶生产在197临床分离的痤疮假单胞杆菌；我们评估了透明质酸酶在三个分离亚群中产生的动力学。方法。采用聚合酶链反应检测透明质酸酶基因。在含有400 μg/mL透明质酸和1%白蛋白的BHI琼脂上培养分离株，用2 N冰醋酸浸水板沉淀未结合的透明质酸，检测透明质酸酶的产生，清除区代表阳性表型。透明质酸酶生产动力学测量作为一个功能的透明质酸消化随时间在液体培养基。结果。在100%和97%的痤疮假单胞杆菌分离株中分别检测到透明质酸酶基因和产透明质酸酶。生长96小时后，在液体培养基中检测到透明质酸酶的产生。结论。透明质酸酶的产生在痤疮假单胞杆菌中几乎是普遍的。在液体培养基中产生大量透明质酸酶似乎需要3天。当从临床标本中分离出痤疮假体时，组织标本中检测透明质酸酶可能是区分痤疮假体感染和定植的一种策略。
Objectives. We sought to describe the prevalence of a hyaluronidase gene and hyaluronidase production in 197 clinical isolates of P. acnes; we assessed kinetics of hyaluronidase production in a subset of three isolates. Methods. The hyaluronidase gene was detected using polymerase chain reaction. Hyaluronidase production was detected by growing isolates on BHI agar containing 400 μg/mL hyaluronic acid and 1% albumin and flooding plates with 2 N glacial acetic acid to precipitate unbound hyaluronic acid, with a zone of clearing representing a positive phenotype. Hyaluronidase production kinetics were measured as a function of hyaluronic acid digestion over time in a liquid medium. Results. A hyaluronidase gene and hyaluronidase production were detected in 100 and 97% of P. acnes isolates, respectively. Hyaluronidase production in liquid medium was detectable after 96 hours of growth. Conclusions. Hyaluronidase production is nearly universal among P. acnes isolates. Three days appear to be required for significant hyaluronidase production in a liquid medium. Detection of hyaluronidase in tissue specimens may be a strategy to differentiate P. acnes infection from colonization when P. acnes is isolated from a clinical specimen.