A ONE-SHOT SOLUTION TO BACTERIAL AND FUNGAL CONTAMINATION IN THE GREEN ALGA CHLAMYDOMONAS REINHARDTII CULTURE BY USING AN ANTIBIOTIC COCKTAIL
A ONE-SHOT SOLUTION TO BACTERIAL AND FUNGAL CONTAMINATION IN THE GREEN ALGA CHLAMYDOMONAS REINHARDTII CULTURE BY USING AN ANTIBIOTIC COCKTAIL
复制标题
使用抗生素混合物一次性解决绿藻莱茵衣藻培养物中的细菌和真菌污染
DOI：
10.1111/j.1529-8817.2010.00904.x
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发表时间：
2010-12-01
影响因子：
2.9
通讯作者：
Pan, Junmin
中科院分区：
文献类型：
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作者：
Kan, Yinan;Pan, Junmin
保持微藻的无菌原种或培养物是微藻生物技术以及基础科研的基础。然而，微藻培养物或原种中细菌和/或真菌的污染通常是一个问题。在这里，我们开发了一种通过使用抗生素混合物来减少或消除细菌和真菌污染的策略。莱茵衣藻该死一种广泛使用的单细胞绿色真菌，已被用作测试生物。氨苄西林，头孢噻肟，和多菌灵的组合去除或减少三种不同的细菌和两种不同的真菌测试的污染。提供了一个循序渐进的程序，这是简单，经济，有效的。
Keeping sterile stocks or cultures of microalgae is fundamental to microalgae biotechnology as well as basic scientific research. However, contamination by bacteria and/or fungi in microalgae cultures or stocks is often a problem. Here, we have developed a strategy for reducing or eliminating bacterial and fungal contamination by using a cocktail of antibiotics. Chlamydomonas reinhardtii P. A. Dang., a widely used unicellular green alga, has been used as a testing organism. A combination of ampicillin, cefotaxime, and carbendazim removed or reduced contamination by three different bacteria and two different fungi tested. A step-by-step procedure is provided, which is simple, economical, and effective.