MICRONEUTRALIZATION TEST FOR INFLUENZA-A AND INFLUENZA-B AND PARA-INFLUENZA-1 AND PARAINFLUENZA-2 VIRUSES THAT USES CONTINUOUS CELL-LINES AND FRESH SERUM ENHANCEMENT
MICRONEUTRALIZATION TEST FOR INFLUENZA-A AND INFLUENZA-B AND PARA-INFLUENZA-1 AND PARAINFLUENZA-2 VIRUSES THAT USES CONTINUOUS CELL-LINES AND FRESH SERUM ENHANCEMENT
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DOI：
10.1128/jcm.12.3.426-432.1980
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发表时间：
1980-01-01
影响因子：
9.4
通讯作者：
CATE, TR
中科院分区：
文献类型：
--
作者：
FRANK, AL;PUCK, J;CATE, TR
开发了针对甲型和乙型流感以及副流感 1 和 2 病毒的改良微量中和测试。使用连续细胞系（Madin-Darby 犬肾和 LLC-MK2）、用于病毒检测的血凝以及转移板和其他微量滴定设备产生了高度机械化的技术，适合轻松且相对便宜地滴定大量血清。通过在测试中添加新鲜仓鼠或人血清，热灭活人血清的滴度提高了 0.5-3.5 log2。新鲜血清增强不会改变血清转化率和血清阴性者的识别，但血清转化的程度往往会增加，阳性滴度的分布也会变宽。对于甲型和乙型流感，微量中和试验中的血清转化率与恒河猴肾管中和试验中获得的血清转化率相当。对于乙型流感，微量中和的血清转化率几乎是血凝抑制试验的两倍，但甲型/苏联（H1N1）流感的血清转化率相似。先前存在的微量中和滴度与对 B 型流感感染的抵抗力密切相关。副流感 1 和 2 的有限经验与之前报告的试管中和试验结果相似。
A modified microneutralization test for influenza A and B and parainfluenza 1 and 2 viruses was developed. Use of continuous cell lines (Madin-Darby canine kidney and LLC-MK2), hemagglutination for virus detection, and transfer plates and other microtiter equipment resulted in a highly mechanized technique suitable for titrating large numbers of sera easily and relatively inexpensively. Titers of heat-inactivated human sera were enhanced 0.5-3.5 log2 by addition of fresh hamster or human serum to the test. Seroconversion rates and identification of seronegative persons were not changed by fresh serum enhancement, but the magnitude of seroconversion was often increased and the distribution of positive titers was broadened. For influenza A and B, seroconversion rates in the microneutralization test were equivalent to those obtained in rhesus monkey kidney tube neutralization tests. For influenza B, seroconversion rates by microneutralization were almost double those obtained with the hemagglutination inhibition test, but the rates were similar for influenza A/USSR (H1N1). Preexisting microneutralization titers correlated well with resistance to infection with influenza B. Limited experience with parainfluenza 1 and 2 was similar to previously reported findings with the tube neutralization test.