Epidermal CCA1 and PMR5 contribute to nonhost resistance in Arabidopsis
Epidermal CCA1 and PMR5 contribute to nonhost resistance in Arabidopsis
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DOI：
10.1093/bbb/zbac160
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发表时间：
2022-10-06
影响因子：
1.6
通讯作者：
Ishikawa, Atsushi
中科院分区：
文献类型：
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作者：
Maeda, Nami;Noguchi, Takaya;Ishikawa, Atsushi
非寄主抗性(NHR)是植物中最强大、最持久的抗性，但对其时空调控知之甚少。生物钟以组织特有的方式发挥作用，并调节植物中的个体生理过程。通过突变和RNA-seq分析，我们揭示了生物钟ASSOCIATED1(CCA1)在NHR对稻瘟病菌组织特异性和时间特异性调控中的作用。稻瘟病菌(Magnaporthe Oryzae)。表皮中CCA1功能的靶向性干扰影响了NHR对拟南芥中稻瘟病菌的时间特异性调节。RNA-SEQ分析表明，接种水稻白粉菌改变了穿透2(Pen2)植株的转录组，并确定PMR5(白粉病抗性5)是CCA1直接靶标的候选基因。在拟南芥pen2pmr5突变体植株中，对稻瘟病菌的一天时间特异性穿透抗性降低。这些发现表明，在拟南芥中，表皮CCA1和PMR5有助于建立对稻瘟病菌具有时间特异性的NHR。
Nonhost resistance (NHR) is the most robust and durable resistance in plants, but its spatiotemporal regulation is poorly understood. The circadian clock functions in a tissue-specific manner and regulates individual physiological processes in plants. Using mutant and RNA-seq analyses, we revealed a role of CIRCADIAN CLOCK ASSOCIATED1 (CCA1) in tissue-specific and time-of-day-specific regulation of NHR to Pyricularia oryzae (syn. Magnaporthe oryzae) in Arabidopsis thaliana (Arabidopsis). Targeted perturbation of CCA1 function in epidermis compromised time-of-day-specific regulation of NHR to P. oryzae in Arabidopsis. RNA-seq analysis showed that P. oryzae inoculation alters the transcriptome in penetration 2 (pen2) plants and identified POWDERY MILDEW RESISTANCE 5 (PMR5) as a candidate gene of direct targets of CCA1. Time-of-day-specific penetration resistance to P. oryzae was reduced in Arabidopsis pen2 pmr5 mutant plants. These findings suggest that epidermal CCA1 and PMR5 contribute to the establishment of time-of-day-specific NHR to P. oryzae in Arabidopsis.